Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : VNV-008
| Cat. No. | VNV-008 |
| Description | Wild type coxsackievirus B5 particles which are prepared from cell cultures and are inactivated by heat treatment. This product is intended for research use only. |
| Storage | -80°C |
| Shipping | Dry ice |
A: To determine the titer of Wild-Type Coxsackievirus B5, a series of dilutions can be prepared from a stock solution of the virus. The dilutions are then inoculated onto appropriate host cells and incubated for a defined period. After incubation, the cells are examined for the presence of viral cytopathic effects (CPE) such as cell rounding, detachment, or lysis. The highest dilution that shows CPE in a significant number of cells is counted as the endpoint. The titer of the virus is calculated by multiplying the endpoint dilution by the dilution factor, representing the reciprocal of the highest dilution. This method provides an estimate of the infectious virus particles in the sample and is commonly used to determine the viral load or concentration.
A: To maintain the viability of Wild-Type Coxsackievirus B5, it is crucial to handle the virus under appropriate biosafety conditions, ensuring that the transmission of the virus is prevented. Proper storage of the virus should be carried out at ultra-low temperatures, preferably below -80°C, to maintain its viability for an extended period. Regular monitoring and documentation of the virus strain, including its isolation source, passage history, and molecular characteristics, should be performed. Additionally, frequent sub-culturing or propagation of the virus in susceptible cells or animal models is necessary to maintain its potency, genetic stability, and pathogenicity. Adhering to these guidelines will help preserve the viability of the Wild-Type Coxsackievirus B5 strain for scientific research, diagnostic purposes, and potential development of therapeutics or vaccines.
A: Coxsackievirus B5 is a strain of the Coxsackievirus B species, which belongs to the Enterovirus genus within the Picornaviridae family.
A: Coxsackievirus infection is diagnosed mainly via serological tests such as ELISA and cell culture.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
The Wild-Type Coxsackievirus B5 offered by Creative Biogene has proved to be a valuable tool in our research. The viral titer provided was optimal, allowing for efficient infection in our cells.
We are delighted with the Wild-Type Coxsackievirus B5 and will continue using it in our future studies."
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.