Cat.No. : VNV-008
Storage: -80°C Shipping: Dry ice
| Cat. No. | VNV-008 |
| Description | Wild type coxsackievirus B5 particles which are prepared from cell cultures and are inactivated by heat treatment. This product is intended for research use only. |
| Shipping | Dry ice |
| Storage | -80°C |
| Creative Biogene ensures high-quality lentivirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between lentivirus particle lots. | |
| Mycoplasma | Creative Biogene routinely tests for mycoplasma contamination using a mycoplasma detection kit. Cell lines are maintained for approximately 20 passages before being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks after thawing, cell culture supernatants are tested for mycoplasma contamination. Creative Biogene ensures that lentiviral products are free of mycoplasma contamination. |
| Purity | Creative Biogene evaluates the level of impurities, such as residual host cell DNA or proteins, in prepared lentiviral vectors to ensure they meet quality standards. |
| Sterility | The lentiviral samples were inoculated into cell culture medium for about 5 days and the growth of bacteria and fungi was tested. Creative Biogene ensures that the lentiviral products are free of microbial contamination. |
| Transducibility | Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of lentivirus to deliver genetic material into target cells, and assess gene expression and functional activities. |
| Proviral Identity Confirmation | All Creative Biogene lentiviral vectors are confirmed to have correctly integrated provirus using PCR. This test involves transducing cells with serial dilutions of the lentiviral vector, harvesting the cells a few days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion of the expected lentiviral insert. |
A: To determine the titer of Wild-Type Coxsackievirus B5, a series of dilutions can be prepared from a stock solution of the virus. The dilutions are then inoculated onto appropriate host cells and incubated for a defined period. After incubation, the cells are examined for the presence of viral cytopathic effects (CPE) such as cell rounding, detachment, or lysis. The highest dilution that shows CPE in a significant number of cells is counted as the endpoint. The titer of the virus is calculated by multiplying the endpoint dilution by the dilution factor, representing the reciprocal of the highest dilution. This method provides an estimate of the infectious virus particles in the sample and is commonly used to determine the viral load or concentration.
A: To maintain the viability of Wild-Type Coxsackievirus B5, it is crucial to handle the virus under appropriate biosafety conditions, ensuring that the transmission of the virus is prevented. Proper storage of the virus should be carried out at ultra-low temperatures, preferably below -80°C, to maintain its viability for an extended period. Regular monitoring and documentation of the virus strain, including its isolation source, passage history, and molecular characteristics, should be performed. Additionally, frequent sub-culturing or propagation of the virus in susceptible cells or animal models is necessary to maintain its potency, genetic stability, and pathogenicity. Adhering to these guidelines will help preserve the viability of the Wild-Type Coxsackievirus B5 strain for scientific research, diagnostic purposes, and potential development of therapeutics or vaccines.
A: Coxsackievirus B5 is a strain of the Coxsackievirus B species, which belongs to the Enterovirus genus within the Picornaviridae family.
A: Coxsackievirus infection is diagnosed mainly via serological tests such as ELISA and cell culture.
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The Wild-Type Coxsackievirus B5 offered by Creative Biogene has proved to be a valuable tool in our research. The viral titer provided was optimal, allowing for efficient infection in our cells.
We are delighted with the Wild-Type Coxsackievirus B5 and will continue using it in our future studies."
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