Cat.No. : VNV-005
Storage: -80°C Shipping: Dry ice
| Cat. No. | VNV-005 |
| Description | Wild-type astrovirus particles and proteins which are prepared from a lysate of host cells and are inactivated by β-propiolactone treatment. This product is intended for research use only. |
| Shipping | Dry ice |
| Storage | -80°C |
| Creative Biogene ensures high-quality lentivirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between lentivirus particle lots. | |
| Mycoplasma | Creative Biogene routinely tests for mycoplasma contamination using a mycoplasma detection kit. Cell lines are maintained for approximately 20 passages before being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks after thawing, cell culture supernatants are tested for mycoplasma contamination. Creative Biogene ensures that lentiviral products are free of mycoplasma contamination. |
| Purity | Creative Biogene evaluates the level of impurities, such as residual host cell DNA or proteins, in prepared lentiviral vectors to ensure they meet quality standards. |
| Sterility | The lentiviral samples were inoculated into cell culture medium for about 5 days and the growth of bacteria and fungi was tested. Creative Biogene ensures that the lentiviral products are free of microbial contamination. |
| Transducibility | Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of lentivirus to deliver genetic material into target cells, and assess gene expression and functional activities. |
| Proviral Identity Confirmation | All Creative Biogene lentiviral vectors are confirmed to have correctly integrated provirus using PCR. This test involves transducing cells with serial dilutions of the lentiviral vector, harvesting the cells a few days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion of the expected lentiviral insert. |
A: In the laboratory, the infection efficiency of Wild-Type Astrovirus can be determined through a variety of experimental techniques. One common method is to infect cultured cells with the virus and measure the percentage of cells that become infected. This can be done by staining the infected cells with specific antibodies that bind to viral proteins, and then using fluorescence microscopy or flow cytometry to quantify the number of infected cells. Alternatively, researchers can also assess the viral replication and spread by measuring the viral RNA or protein levels in the infected cells using molecular biology techniques like PCR or Western blotting. These techniques provide quantitative data on the infection efficiency of Wild-Type Astrovirus and help researchers understand its pathogenicity and potential for spreading in vivo.
A: To detect the purity of Wild-Type Astrovirus, several methods can be employed. Firstly, viral isolation from various clinical samples can be performed using cell cultures or animal models. Once isolated, the virus can be confirmed as Wild-Type Astrovirus through genetic sequencing and comparison with known strains. Additionally, electron microscopy can be used to visualize the virus particles, confirming their morphology and size. Immunological methods such as enzyme-linked immunosorbent assay (ELISA) or immunofluorescence can be employed to detect specific viral proteins or antigens, providing further evidence of purity. These techniques, in combination, can ensure the accurate detection and identification of Wild-Type Astrovirus in clinical samples.
A: Astrovirus is a type of single-stranded RNA virus that infects a variety of animals, including humans. It was first discovered in 1975, when it was found in stool samples from children suffering from diarrhea.
A: Astroviruses have a star-like appearance with five or six points. They are non-enveloped RNA viruses with cubic capsids, approximately 28–35 nm in diameter with T=3 symmetry.
A: The genome of Astrovirus is between 6.8-7.9 kb long. The genome is arranged into three open reading frames (ORFs), with an overlap of approximately 70 nucleotides between ORF1a and ORF1b. The remaining ORF is known as ORF2.
A: Astroviruses can cause gastroenteritis, with symptoms including diarrhea, vomiting, abdominal pain, and fever. In most cases, the infection is self-limiting and resolves within a few days. However, severe cases may occur in people with weakened immune systems, young children, and the elderly.
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The virus samples received have consistently shown high viability and infectivity, enabling us to effectively study the mechanisms of Astrovirus replication and its interactions with host cells.
The comprehensive documentation accompanying the products has been immensely valuable, providing detailed protocols and instructions that have greatly enhanced our experiments.
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