Tet-On GFP Stable Cell Line - HEK293

Name: Tet-On GFP Stable Cell Line - HEK293
SKU: CSC-RR0573
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RR0573

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

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Cat. No.	CSC-RR0573
Description	The overexpression cassette containing GFP reporter gene driven by a tetracycline inducible promoter (Tet-On system) was stably integrated into the HEK293 genome. GFP expression is rapidly induced in the presence of tetracycline (Tc) or tetracycline-derivatives like doxycycline (dox).
Target Gene	Tet-On GFP
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

CSC-RR0573-Tet-On GFP Stable Cell Line - HEK293-Datasheet

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The Tet-On GFP Stable Cell Line is a genetically modified HEK293 cell line that allows for the inducible expression of the Green Fluorescent Protein (GFP). The Tet-On system is a tetracycline-based regulatory system that enables the controlled expression of genes in response to the presence of tetracycline or its derivatives.

The HEK293 cell line, derived from human embryonic kidney, is a widely used model for studying gene expression and protein function. The Tet-On GFP Stable Cell Line is particularly useful for studying the dynamics of protein expression and localization in living cells, as well as for drug screening applications where the visualization of cellular processes is required.

In the realm of cellular biology, the intricate interactions between intracellular proteins play pivotal roles in both normal physiology and various diseases. Traditional methods aimed at modifying these pathological protein-protein interactions often face significant challenges. Researchers are now turning to innovative solutions like bioPROTACs—engineered proteins that facilitate targeted degradation of therapeutic targets by fusing a target-binding domain with an E3 ligase. This study highlights the adaptability of bioPROTACs in targeting proteins such as proliferating cell nuclear antigen (PCNA), showcasing their potential for therapeutic applications and biological research.

Figure 1 describes a flow cytometric analysis demonstrating the flexibility of different GFP binders used to generate bioPROTACs, highlighting their efficacy in depleting H2B-GFP in HEK293 Tet-On 3G cells. (doi: 10.1073/pnas.1920251117) Figure 1. The researchers employed flow cytometric analysis using H2B-GFP/HEK293 Tet-On 3G cells to evaluate the efficacy of various GFP binders. By inducing mCherry expression with doxycycline, they aimed to assess the flexibility of bioPROTAC design in facilitating targeted protein degradation. (Lim S, et al., 2020)

Creative Biogene's Tet-On GFP Stable Cell Line - HEK293 offers a versatile platform for similar targeted degradation studies. With its ability to stably express GFP-tagged proteins, this cell line enables researchers to easily visualize and analyze protein interactions and degradation processes.

The Tet-On GFP Stable Cell Line - HEK293 is a cell line that allows for the inducible expression of the green fluorescent protein (GFP) under the control of a tetracycline-responsive promoter. This system enables researchers to tightly regulate gene expression, making it a versatile tool for various applications.

（1）Gene Expression Studies:

The Tet-On system allows for the controlled expression of genes of interest, making it ideal for studying the effects of GFP on cell behavior, localization, and function without the constant need for the inducer, tetracycline.

（2）Cell Signaling and Trafficking:

By using the Tet-On GFP Stable Cell Line, researchers can visualize and track the dynamics of protein localization and intracellular trafficking in real-time, providing valuable insights into cellular processes.

（3）Drug Screening and Toxicity Assessment:

The inducible expression of GFP can be used to assess the toxicity of compounds by monitoring changes in fluorescence, which can indicate cellular stress or damage.

Customer Q&As

How does the Tet-On GFP Stable Cell Line - HEK293 enable researchers to dynamically control GFP expression and what are the implications for studying protein localization and trafficking?

A: The Tet-On GFP Stable Cell Line - HEK293 allows researchers to control the expression of GFP through the addition or removal of tetracycline or its derivatives, such as doxycycline. This system enables the study of protein localization and trafficking by providing a means to temporally regulate GFP fusion proteins. Researchers can observe the effects of GFP expression on cellular processes without the need for labor-intensive and time-consuming protein tagging for each individual study, thus streamlining the research process and allowing for more dynamic analysis of protein behavior within living cells.

In what ways can the Tet-On GFP Stable Cell Line - HEK293 be utilized to investigate the effects of gene expression on cell cycle regulation and cell proliferation?

A: The Tet-On GFP Stable Cell Line - HEK293 can be engineered to express genes of interest under the control of the inducible promoter. By inducing GFP expression, researchers can monitor cell cycle progression and proliferation rates in real-time. The system can be further adapted to express proteins that are known to affect cell cycle regulation, allowing for a detailed analysis of how these proteins influence cell cycle dynamics. This approach can lead to a better understanding of the molecular mechanisms that govern cell growth and division.

How can the Tet-On GFP Stable Cell Line - HEK293 contribute to the development of gene therapy strategies by providing insights into the safety and efficacy of inducible gene expression systems?

A: The Tet-On GFP Stable Cell Line - HEK293 serves as an excellent model for evaluating the safety and efficacy of inducible gene expression systems for potential gene therapy applications. By using this cell line, researchers can test the responsiveness of the inducible promoter system to various inducers and assess the stability and long-term expression of the transgene. Additionally, the system can be used to evaluate potential off-target effects and the impact of gene expression on cellular function, which are critical considerations for the development of safe and effective gene therapies.

What role can the Tet-On GFP Stable Cell Line - HEK293 play in the study of gene regulation and epigenetic modifications under different cellular conditions?

A: The Tet-On GFP Stable Cell Line - HEK293 can be utilized to investigate how different cellular conditions, such as stress, differentiation, or exposure to various chemical agents, affect gene regulation and epigenetic modifications. By inducing GFP expression and monitoring changes in chromatin structure and histone modifications, researchers can gain insights into the epigenetic mechanisms that control gene expression. This knowledge can contribute to the understanding of how environmental factors influence gene regulation and cellular behavior.

How might the Tet-On GFP Stable Cell Line - HEK293 be employed to develop and optimize inducible expression systems for use in biotechnology and industrial applications?

A: The Tet-On GFP Stable Cell Line - HEK293 can be used to fine-tune inducible expression systems for biotechnological and industrial purposes. By testing the responsiveness of the cell line to different inducers and varying concentrations, researchers can optimize the expression system for maximum efficiency and minimal toxicity. This cell line can also be used to screen for novel inducers or to engineer cells with improved inducible expression capabilities, which can be beneficial for the production of recombinant proteins or the development of cell-based biosensors.

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Customer Reviews

Inducible GFP Expression

The Tet-On GFP Stable Cell Line - HEK293 offers inducible GFP expression, allowing us to control when GFP is expressed by adding or removing doxycycline. This control is crucial for temporal studies where the timing of gene expression is critical to the experimental outcomes.

French

2023-06-08

Visual Tracking of Gene Expression

With GFP as a reporter, the Tet-On GFP Stable Cell Line - HEK293 enables visual tracking of gene expression. This visualization is invaluable for confirming the presence and location of gene expression in real time, facilitating immediate observational studies.

French

2022-07-06

High Transfection Efficiency

HEK293 cells are known for their high transfection efficiency, making the Tet-On GFP Stable Cell Line - HEK293 ideal for introducing additional genetic materials. This characteristic is beneficial for experiments requiring the co-expression of other genes alongside GFP.

United States

2024-02-28

Robust for Cell

The Tet-On GFP Stable Cell Line - HEK293 benefits from the robust growth and easy maintenance characteristics of HEK293 cells, which supports a wide range of experimental conditions and reduces the complexity of cell culture management.

United States

2022-01-15

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Tet-On GFP Stable Cell Line - HEK293

Cell Line Information

Cell Culture Information

Safety and Packaging

Documents

Background

Case Study

Applications

Q & A

Customer Reviews

How does the Tet-On GFP Stable Cell Line - HEK293 enable researchers to dynamically control GFP expression and what are the implications for studying protein localization and trafficking?

In what ways can the Tet-On GFP Stable Cell Line - HEK293 be utilized to investigate the effects of gene expression on cell cycle regulation and cell proliferation?

How can the Tet-On GFP Stable Cell Line - HEK293 contribute to the development of gene therapy strategies by providing insights into the safety and efficacy of inducible gene expression systems?

What role can the Tet-On GFP Stable Cell Line - HEK293 play in the study of gene regulation and epigenetic modifications under different cellular conditions?

How might the Tet-On GFP Stable Cell Line - HEK293 be employed to develop and optimize inducible expression systems for use in biotechnology and industrial applications?

Inducible GFP Expression

Visual Tracking of Gene Expression

High Transfection Efficiency

Robust for Cell

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