Cat.No. : AAB0017
Titer: ≥1x10^12 GC/mL / ≥1x10^13 GC/mL Size: 30 ul/100 ul/500 ul/1 ml
Serotype: AAV Serotype 9 Storage: -80 ℃
| Cat. No. | AAB0017 |
| Description | Premade AAV particles in serotype 9 containing Cre-dependent jCaMP7b under the control of a Syn promoter. |
| Serotype | AAV Serotype 9 |
| Tag | jGCaMP7b |
| Product Type | Adeno-associated virus particles |
| Biosensor | jGCaMP7b-Improved SNR, higher baseline fluorescence |
| Titer | Varies lot by lot, typically ≥1x10^12 GC/mL |
| Size | Varies lot by lot, for example, 30 μL, 50 μL, 100 μL etc. |
| Storage | Store at -80℃. Avoid multiple freeze/thaw cycles. |
| Shipping | Frozen on dry ice |
| Creative Biogene ensures high-quality AAV particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between AAV particle lots. | |
| Endotoxin | Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in the production of AAV, especially for applications in animal studies and gene therapy. Effective endotoxin quality control is essential in the development and manufacturing of AAV particles. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced AAV particles to ensure regulatory compliance. |
| Purity | AAV purity is critical for ensuring the safety and efficacy of AAV-based applications.AAV capsids are composed of three main protein components, known as viral proteins: VP1, VP2, and VP3. These proteins play a critical role in the structure and functionality of the AAV capsid. Monitoring the VP1, VP2, and VP3 content in AAV preparations is essential for quality control in AAV production. Our AAV particles are tested for showing three clear bands of VP1, VP2 VP3 by SDS-PAGE. |
| Sterility | The AAV virus samples are inoculated into the cell culture medium for about 5 days to detect bacterial and fungal growth. |
| Transducibility | Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of AAV to deliver genetic material into target cells or tissues, and assess gene expression and functional activities. |
| Empty vs. Full Capsids | Based-on our proprietary AAV production and purification technology, Creative Biogene can always offer AAV particles with high ratio of full capsids. If required, we can also assess the ratio for a specifc lot of AAV particles by transmission electron microscopy (TEM) or other methods. |
Animals can learn to repeat behaviors in order to obtain desired rewards, a process often called reinforcement learning. Although previous studies have implicated ascending dopaminergic projections to the basal ganglia in reinforcement learning, little is known about the role of the hippocampus. Here, it was shown that specific populations of hippocampal neurons and their dopaminergic innervation contribute to operant self-stimulation. These neurons are located in the dentate gyrus, receive dopaminergic projections from the locus coeruleus, and express D1 dopamine receptors. Activating D1+ dentate neurons is sufficient for self-stimulation: mice press a lever to obtain optogenetic activation of these neurons. Similar effects were observed with selective activation of locus coeruleus projections to the dentate gyrus and blockade by D1 receptor antagonists. Calcium imaging of D1+ dentate neurons revealed significant activity during action selection but not during passive reward delivery. These results reveal a role for hippocampal dopaminergic innervation in supporting operant reinforcement.
Researchers implanted a gradient index lens above the DG of D1-Cre mice (N = 5) and injected the mice with a Cre-dependent calcium indicator (AAV9-syn-FLEX-jGCamp7f) (Figure 1A- B). Then, calcium transients in DG D1+ neurons during lever pressing to obtain food reward were recorded on a fixed-ratio (FR) schedule (FR1, FR3, and FR5) (Figure 1A-C). Study identifies distinct populations of DG D1+ neurons modulated by lever pressing. To see if the neural activity is action-contingent, a control task that did not require pressing was also used. Rewards were delivered non-contingently every 20 seconds, preceded by a second of white noise. In this task, compared to the operant task, far fewer DG D1+ neurons (N = 6, 3% of the total population) were significantly modulated (Figure 1F).
Figure 1. D1+ dentate gyrus neurons are significantly modulated by lever pressing during operant conditioning. (Petter E A, et al., 2022)
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Each batch we used was reliable, with no variability between experiments, which spared us from unexpected troubleshooting and allowed us to focus on data analysis.
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