Syn-FLEX-CaMPARI AAV (Serotype 9)

Name: Syn-FLEX-CaMPARI AAV (Serotype 9)
SKU: AAB0011
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : AAB0011

Serotype : AAV Serotype 9 Storage : -80 ℃

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Virus Particles Information

Quality Control

Cat. No.	AAB0011
Description	Premade AAV particles in serotype 9 containing Cre-dependent CaMPARI under the control of a Syn promoter.
Product Type	Adeno-associated virus particles
Tag	CaMPARI
Serotype	AAV Serotype 9
Biosensor	CaMPARI-High-throughput calcium assays with cultured cells
Titer	Varies lot by lot, typically ≥1x10^12 GC/mL
Size	Varies lot by lot, for example, 30 μL, 100 μL, 500 μL etc.
Storage	Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping	Frozen on dry ice

Summary	Creative Biogene ensures high-quality AAV particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between AAV particle lots.
Endotoxin	Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in the production of AAV, especially for applications in animal studies and gene therapy. Effective endotoxin quality control is essential in the development and manufacturing of AAV particles. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced AAV particles to ensure regulatory compliance.
Purity	AAV purity is critical for ensuring the safety and efficacy of AAV-based applications.AAV capsids are composed of three main protein components, known as viral proteins: VP1, VP2, and VP3. These proteins play a critical role in the structure and functionality of the AAV capsid. Monitoring the VP1, VP2, and VP3 content in AAV preparations is essential for quality control in AAV production. Our AAV particles are tested for showing three clear bands of VP1, VP2 VP3 by SDS-PAGE.
Sterility	The AAV virus samples are inoculated into the cell culture medium for about 5 days to detect bacterial and fungal growth.
Transducibility	Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of AAV to deliver genetic material into target cells or tissues, and assess gene expression and functional activities.
Empty vs. Full Capsids	Based-on our proprietary AAV production and purification technology, Creative Biogene can always offer AAV particles with high ratio of full capsids. If required, we can also assess the ratio for a specifc lot of AAV particles by transmission electron microscopy (TEM) or other methods.

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In vivo gene therapy offers the possibility of a one-time cure for genetic diseases with the promise of lifelong beneficial effects. Gene therapy mediated by recombinant adeno-associated virus (rAAV) vectors has shown great promise in multiple clinical trials for neurological diseases, with sustained transgene expression and functional responses, as well as safety. While rAAV serotype 2 (rAAV2) has been the most widely used in clinical trials, many other serotypes have shown enhanced ability to transduce neurons in experimental studies.

The adeno-associated virus serotype 9 (AAV9) vector has generated considerable interest in its therapeutic development for the treatment of neurological diseases, in part because of its perceived ability to cross the blood-brain barrier to target cells in the central nervous system (CNS). These cells include endothelial cells, neurons and astrocytes in the brain, and motor neurons and astrocytes in the spinal cord. The ability of AAV9 to target these CNS cells makes AAV9-mediated gene correction a viable therapy for ALD/AMN, as long bundles of CNS axons and their supporting glial cells are the site of pathology.

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Customer Reviews

Reliable Consistency

I’m truly impressed with the consistency of the Syn-FLEX-CaMPARI AAV (Serotype 9). Every batch we’ve used has delivered reliable results, ensuring that our experiments can proceed without unexpected delays.

Canada

2023-12-01

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Syn-FLEX-CaMPARI AAV (Serotype 9)

Virus Particles Information

Quality Control

Background

Q & A

Customer Reviews

Reliable Consistency

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