Pseudovirus Service

The COVID-19 pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is threatening people's health around the world. The virus had already arrived in numerous areas of the world due to its potent transmissibility. As the virus continues its global spread, understanding of SARS-CoV-2 mechanism is critical for drug discovery and vaccine development. As a leading company, Creative Biogene provides reliable and high-quality pseudovirus service in support of SARS-CoV-2 studies. We will make our own contribution to fight against COVID-19 disease.

pseudovirus-service

COVID-19 crises have led to enormous worldwide efforts to urgently develop drug treatments and vaccines effective against SARS-CoV-2. Due to the high infectivity and pathogenicity, SARS-CoV-2 should be dealt with in biosafety level 3 laboratories, which limit the anti-viral measure development. Pseudoviruses have the essential components for cell entry and viral infection, but lack of nucleic acid and lose self-replication ability. They have been regarded as the effective virological tools due to their safety and versatility, especially for appearing and re-appearing viruses. At present, researchers use pseudoviruses to mimic SARS-CoV-2 virus for further studies.

SARS-CoV-2 has four main structural proteins. The S protein is one of the key components to bind the receptor and mediate membrane fusion and virus entry. It is a main target for neutralizing antibodies and vaccine design. With extensive experience in pseudoviruses, Creative Biogene provides pseudovirus service based on lentiviral and VSV systems for SARS-CoV-2 studies. We have developed a pseudotype system with S protein of SARS-CoV-2 to support virus entry study in BSL2 facilities. Pseudovirus-based neutralization assays are provided for detecting neutralizing antibodies. In addition, multiple related pseudovirus products and custom services are also available.

The following is the details of pseudoviruses provided by Creative Biogene:

Cat. No.	Product Name	Price
CoV-001	SARS-CoV-2 S Pseudotyped GFP Lentivirus	Inquiry
CoV-001a	SARS-CoV-2 S Pseudotyped GFP Lentivirus (Alpha strain S)	Inquiry
CoV-001b	SARS-CoV-2 S Pseudotyped GFP Lentivirus (Beta strain S)	Inquiry
CoV-001d	SARS-CoV-2 S Pseudotyped GFP Lentivirus (Delta strain S)	Inquiry
CoV-001o	SARS-CoV-2 S Pseudotyped GFP Lentivirus (Omicron strain S)	Inquiry
CoV-002	SARS-CoV-2 S Pseudotyped Luciferase Lentivirus	Inquiry
CoV-002a	SARS-CoV-2 S Pseudotyped Luciferase Lentivirus (Alpha strain S)	Inquiry
CoV-002b	SARS-CoV-2 S Pseudotyped Luciferase Lentivirus (Beta strain S)	Inquiry
CoV-002d	SARS-CoV-2 S Pseudotyped Luciferase Lentivirus (Delta strain S)	Inquiry
CoV-002o	SARS-CoV-2 S Pseudotyped Luciferase Lentivirus (Omicron strain S)	Inquiry
CoV-009	SARS-CoV-2 S Pseudotyped mCherry Lentivirus	Inquiry
CoV-010	SARS-CoV2 S-ΔG-mCherry Pseudotyped VSV	Inquiry
CoV-012	SARS-CoV2 S-ΔG-GFP Pseudotyped VSV	Inquiry
CoV-013	SARS-CoV2 S-ΔG-Luciferase Pseudotyped VSV	Inquiry
CoV-014	SARS-CoV2 S-ΔG Pseudotyped VSV	Inquiry
CoV-015	SARS-CoV-2 S Pseudotyped GFP-Luciferase Lentivirus	Inquiry
CoV-016	SARS-CoV-2 S D614G Pseudotyped GFP-Luciferase Lentivirus	Inquiry
CoV-017	SARS-CoV-2 S D614G Pseudotyped mCherry-Luciferase Lentivirus	Inquiry
CoV-018	SARS-CoV-2 S E484K/N501Y Pseudotyped GFP Lentivirus	Inquiry
CoV-019	SARS-CoV-2 S E484K/N501Y Pseudotyped Luciferase Lentivirus	Inquiry
CoV-020	SARS-CoV-2 S N501Y Pseudotyped GFP Lentivirus	Inquiry
CoV-021	SARS-CoV-2 S N501Y Pseudotyped GFP-Luciferase Lentivirus	Inquiry

Why Choose Creative Biogene

Mature and excellent platform
Years of experience in pseudovirus
Rapid turnaround time
Competitive price
Customized pseudovirus products and services

Together with global researchers and scientists, Creative Biogene is dedicating to provide pseudovirus service with high quality in support of SARS-CoV-2 research. More pseudoviruses are available at Creative Biogene. If you are interested in our pseudovirus service or have any additional requirements, please feel free to contact us.

In order to better support SARS-CoV-2 studies, related virus particles are provided in the table below:

Cat.No.	Product Name
AD00537Z	Human ACE2 adenoviral particles
LV04219L	human ACE2 (NM_021804) lentivirus particles
AD16629Z	Human TMPRSS2 adenoviral particles
LV28259L	human TMPRSS2 (NM_005656) lentivirus particles
LV28260L	human TMPRSS2 (NM_001135099) lentivirus particles

References:

Nie, J., Li, Q., Wu, J., Zhao, C., Hao, H., Liu, H., Zhang, L., Nie, L., Qin, H., Wang, M., Lu, Q., Li, X., Sun, Q., Liu, J., Fan, C., Huang, W., Xu, M., Wang, Y. (2020). Establishment and validation of a pseudovirus neutralization assay for SARS-CoV-2. Emerging Microbes & Infections, 9(1), 680–686.
Walls, A.C., Park, Y.J., Tortorici, M.A., Wall, A., McGuire, A.T., Veesler, D. (2020) Structure, Function, and Antigenicity of the SARS-CoV-2 Spike Glycoprotein. Cell. 181(2):281-292.e6.
Ou, X., Liu, Y., Lei, X., Li, P., Mi, D., Ren, L., Guo, L., Guo, R., Chen, T., Hu, J., Xiang, Z., Mu, Z., Chen, X., Chen, J., Hu, K., Jin, Q., Wang, J.,Qian, Z. (2020). Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and its immune cross-reactivity with SARS-CoV. Nature Communications, 11(1).

Publications

Li Z, Zhang X Q, Ho W, et al. Lipid‐Polymer Hybrid “Particle‐in‐Particle” Nanostructure Gene Delivery Platform Explored for Lyophilizable DNA and mRNA COVID‐19 Vaccines[J]. Advanced Functional Materials, 2022, 32(40): 2204462.
Almeida-da-Silva C L C, Matshik Dakafay H, Liu K, et al. Cigarette smoke stimulates SARS-CoV-2 internalization by activating AhR and increasing ACE2 expression in human gingival epithelial cells[J]. International journal of molecular sciences, 2021, 22(14): 7669.
Cutone A, Rosa L, Bonaccorsi di Patti M C, et al. Lactoferrin binding to SARS-CoV-2 spike glycoprotein blocks pseudoviral entry and relieves iron protein dysregulation in several in vitro models[J]. Pharmaceutics, 2022, 14(10): 2111.

* For research use only. Not intended for any clinical use.

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