pTV118N phagemid vector

The pTV118N is a hybrid phagemid vector that is constructed from the modified pUC118 plasmid. This specialized vector is distinguished by the presence of a CCATGG sequence, which serves dual functions. Firstly, it incorporates the cleavage site for the NcoI restriction enzyme, enabling targeted dissection of genetic material at this specific site. Secondly, the sequence encompasses the start codon ATG for the lacZ gene that permits commencement of specific gene expression. Altogether, this facilitates the specific expression of any target gene at the NcoI site. Notable, the pTV118N vector operates through the lac promoter system, which is incorporated into its design. This lac promoter system plays a pivotal role in enabling efficient protein expression. Furthermore, this vector includes a lacZ Shine-Dalgarno (SD) sequence. The lacZ SD sequence is positioned eight bases from the initiation codon, creating an optimal context for the high-level expression of target genes. Another key feature of the pTV118N vector that contributes to its utility is the mechanism for the induction of single-stranded DNA by a helper phage. This innovative approach allows the accurate sequencing of the start codon site. The inclusion of the RV-N primer ensures precise sequencing by starting it from the start codon site. This capability secures the correct translation frame of an insert. This ability to determine the exact nucleotide sequence of DNA fragments from the start codon site enhances the accuracy and efficacy of genetic engineering applications.