Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : VPT4038
| Cat. No. | VPT4038 |
| Description | The λCre-Blue Vector enables autosubcloning by Cre recombinase-mediated excision. The native vector carries a 13,253-bp stuffer insert containing lacZ and inert EMC viral and lambda sequences. The pCre-Blue plasmid derived from the native vector carries the 13,253-bp stuffer, and has a blue colony phenotype when plated in the presence of Xgal due to the presence of the lacZ gene. |
| Vector Type | phagemid vector |
The λCre-Blue Vector is a phagemid vector specifically designed to facilitate autosubcloning through the process of Cre recombinase-mediated excision. The vector carries a 13,253-bp stuffer insert which includes a lacZ gene and inert EMC viral as well as lambda sequences. This elaborate mechanism is streamlined to ensure efficient excision and subcloning capabilities.
The pCre-Blue plasmid exhibits a blue colony phenotype upon cultivation. The presence of the lacZ gene enables the process of beta-galactosidase production, a key enzyme responsible for cleaving lactose into simpler sugars. The enzyme’s activity turns Xgal into a blue product, hence the observed blue colonies. Therefore, the λCre-Blue Vector is an essential resource in biological experimentation and genetic modification, serving as a reliable and effective catalyst for Cre recombinase-mediated excision.
The λCre-Blue Vector is an invaluable tool in molecular biology research, allowing for more efficient and precise gene alterations. Using Cre-recombinase, this vector enables autosubcloning, a method in which a segment of DNA is automatically replicated and inserted back into the genome. This brings with it many applications, making it an ideal tool for creating knockout mutations or the introduction of new genes.
Firstly, it is used extensively in knockout studies. These investigations involve the deactivation, or 'knocking out', of specific genes within an organism to understand its function. The λCre-Blue vector simplifies this process significantly by allowing for the targeted removal of these genes.
Because the λCre-Blue vector allows for the precise insertion of new genes into an organism's genome, it is particularly useful in the development of gene-therapeutic interventions.
Additionally, the λCre-Blue vector can be used in the creation of transgenic organisms, which have had their genetic make-up altered by the addition of foreign genes.
In conclusion, the λCre-Blue vector offers a high degree of manipulation over an organism's genetic code, making it a vital tool in researching genetic function, developing therapies for genetic disorders and creating transgenic organisms.
A: The main advantage of phagemid vectors is their ability to produce single-stranded DNA (ssDNA) copies, which can be used for DNA sequencing and other applications. The ssDNA can be synthesized using the bacteriophage's replication functions, resulting in a higher yield of the desired DNA template.
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I am very happy with the λCre-Blue carrier. Its ability to facilitate automated subcloning via Cre recombinase-mediated excision greatly simplifies the entire process.
The Cre recombinase-mediated excision process is quite smooth and streamlined. I've noticed significant improvement in my experiments.
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