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pADL-M13 display Vector

Cat.No.
VPT4029
Description
pADL-M13 is a phagemid vector designed for phage display on the filamentous bacteriophage M13 or equivalent. This vector contains a PelB leader sequence followed by a full-length copy of the gene III sequence and a double-SfiI cloning site to introduce foreign polypeptides on the N-terminal side of the gene III protein. Expression of the fusion is under the tight control of a lac promoter. A strong transcriptional terminator upstream from the promoter efficiently represses undesirable expression and prevents promoter leakiness in absence of induction, thus limiting negative selection against clones bearing toxic products. The absence of amber codon before the gene III results in strong display.
Promoter
LacZ
Resistance
Amp
Selection
Amp
Vector Length
4,990 bp
Vector Map
Vector Type
phagemid vector

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