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pADL-M13 display Vector

For research use only. Not intended for any clinical use.
Cat.No.
VPT4029
Description
pADL-M13 is a phagemid vector designed for phage display on the filamentous bacteriophage M13 or equivalent. This vector contains a PelB leader sequence followed by a full-length copy of the gene III sequence and a double-SfiI cloning site to introduce foreign polypeptides on the N-terminal side of the gene III protein. Expression of the fusion is under the tight control of a lac promoter. A strong transcriptional terminator upstream from the promoter efficiently represses undesirable expression and prevents promoter leakiness in absence of induction, thus limiting negative selection against clones bearing toxic products. The absence of amber codon before the gene III results in strong display.
Promoter
LacZ
Resistance
Amp
Selection
Amp
Vector Length
4,990 bp
Vector Type
phagemid vector
Vector Map

Background

Applications

Publications

Q & A

Customer Reviews

pADL-M13 is a specialized phagemid vector constructed for phage display on filamentous bacteriophage M13, or any phage equivalent to it. The vector encompasses a PelB leader sequence trailed by a full-length copy of Gene III's sequence and a dual SfiI cloning site. This allows users to embed foreign polypeptides on the N-terminal section of the Gene III protein. Regardless of the fusion generated, its expression level is tightly controlled by the lac promoter. The pADL-M13 also includes a powerful transcriptional terminator sited upstream from the promoter. In addition, the absence of an amber codon before the Gene III significantly boosts display potential, offering researchers with additional capabilities in their experimental design. This noteworthy attribute further underscores the precision and efficiency of the pADL-M13 vector. The pADL-M13 adheres to ampicillin resistance selection criteria, thereby simplifying the tracking and propagation of bacteria that harbor the plasmid.
The pADL-M13 Phage Display Vector has diverse applications, mainly in biomedical research, molecular biology, and genetic engineering disciplines. Here are some of its primary uses: Antibody Library Construction: The pADL-M13 vector is widely used to construct phage display libraries of antibodies, especially for human antibodies. These libraries are powerful tools for identifying novel antibodies and engineering antibody fragments with high therapeutic value. Peptide Screening: This vector can be used to screen for high-affinity peptides that bind to a specific target protein. This application is significant in drug discovery and development. Protein-Protein Interaction Studies: The pADL-M13 vector is commonly utilized in protein-protein interaction studies. Researchers can identify the interaction between different proteins, which is crucial in understanding various cellular processes and disease pathogenesis. Vaccine Development: The ability of pADL-M13 to display foreign peptides on the surface of filamentous phage makes it an excellent tool for vaccine development. The displayed antigen can elicit an immune response, hence aiding in vaccine production.
Customer Q&As
What is pADL-M13 vector?

A: pADL-M13 is a phagemid vector designed for phage display on the filamentous bacteriophage M13 or its equivalents. It can be used to facilitate the presentation of foreign polypeptides on the surface of the bacteriophage for various applications, such as peptide or protein interaction studies.

What key features does the pADL-M13 vector include?

A: The pADL-M13 vector includes a PelB leader sequence, a full-length copy of the gene III sequence, and a double-SfiI cloning site. These features allow the insertion of foreign polypeptides on the N-terminal side of the gene III protein, enabling effective display on the phage surface.

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Customer Reviews
Easy to use

The pADL-M13 Phage Display Vector is of high quality and superb efficiency. As a researcher, I found this vector quite easy to use in my experiments.

United States

12/11/2021

Save time and effort

The pADL-M13 Display Vector has helped me to save time and effort, which I was able to devote to further research. I'd highly recommend the pADL-M13 phage display vector to fellow researchers in the field.

United Kingdom

08/23/2020

Worked very well

pADL-M13 Display Vector has enhanced the speed and accuracy of my work, providing consistent and reproducible results every time.

Canada

05/02/2020

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