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pEZZ 18-proA Vector

For research use only. Not intended for any clinical use.
Cat.No.
VPT4033
Description
The phagemid pEZZ 18-proA contains the protein A signal sequence and two synthetic "Z" domains based on the "B" IgG binding domain of protein A. Proteins are expressed as fusions with the "ZZ" peptide and secreted into the aqueous culture medium under the direction of the protein A signal sequence. They are easily purified using IgG Sepharose 6 Fast Flow to which the "ZZ" domain binds tightly. Because of its folding properties, the 14 kDa "ZZ" peptide has little effect on folding of the fusion partner into a native conformation.
Host Cell
E. coli strains carrying a lac deletion but capable of a-complementation of lacZ'
Promoter
LacZ
Resistance
70 μg/ml ampicillin
Selection
70 μg/ml ampicillin
Source
E. coli strains carrying a lac deletion but capable of a-complementation of lacZ'
Vector Length
4,591bp
Vector Type
phagemid vector
Vector Map

Background

Applications

Publications

Q & A

Customer Reviews

The pEZZ 18-proA Vector is a phagemid type vector with a length of 4,591 base pairs. This vector stands out due to its intricately designed structure that includes the protein A signal sequence and two synthetic "Z" domains. These "Z" domains are typically based on the "B" IgG binding domain of protein A, enabling proteins to be expressed as fusions with the "ZZ" peptide. The protein A signal sequence is responsible for directing the secreted proteins into the aqueous culture medium. Moreover, the pEZZ 18-proA Vector allows the purification of these proteins to be significantly simplified by using IgG Sepharose 6 Fast Flow. The said "ZZ" domain binds tightly to this, making the process very efficient. Unique to this vector is the 14 kDa "ZZ" peptide which, because of its favorable folding properties, hardly disrupts the folding of the fusion partner into its native conformation. This ensures that the proteins maintain their original, biologically active structure even after expression and purification.
The pEZZ 18-proA Vector is fundamentally an indispensable tool used in the field of molecular biology studies. Unlike plasmids, phagemids possess both bacteriophage and plasmid characteristics. This inimitable nature is ideal for cloning and sequencing applications as it allows for the potential replication of the phagemid to create massively high copy numbers in a host. The applications of pEZZ 18-proA Vector significantly include gene expression studies. Since the vector permits gene fusion with protein A, the product's expression in the host organism allows for underpinning the function and structure of the gene product. This is key, especially for novel genes, where it can realize their potential roles in disease progression and possible therapeutic interventions. Furthermore, the pEZZ 18-proA Vector is efficiently deployed in antibody screening, considering its capacity to generate fusion proteins with phage enzymes. It provides an amplified library for screening potential antibodies with high affinity, proving instrumental in generating monoclonal antibodies for disease diagnosis and treatment. Besides, the pEZZ 18-proA Vector finds great utilization in protein-protein interaction studies. The vector's potential to generate fusion proteins makes it excellent for studying protein interactions, especially in tandem affinity purification (TAP) technique, defining intricate cellular pathways and identifying potential therapeutic targets.
Customer Q&As
How is the pEZZ 18-proA vector expressed?

A: Expression is controlled by the lacUV5 and protein A promoters and is not inducible.

How is the pEZZ 18-proA vector clone?

A: Inserts containing a stop codon will yield white colonies when grown on media containing X-gal.

What is the host of pEZZ 18-proA vector?

A: E. coli strains carrying a lac deletion but capable of a-complementation of lacZ’.

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Customer Reviews
Easy to use

The pEZZ 18-proA Vector is incredibly easy to use and manipulate. As a biotechnology student, it has certainly simplified my studying and research.

Germany

03/14/2024

Save time

I've found the pEZZ 18-proA Vector to be remarkably efficient for my lab work. Its high copy number significantly speeds up the production of my plasmids, making it a highly reliable phagemid vector.

Germany

05/12/2022

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