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pCDisplay-3M vector

For research use only. Not intended for any clinical use.
Cat.No.
VPT4022
Host Cell
TG1
Promoter
M13,ColE
Resistance
Amp
Selection
Ampicillin
Tag
His6,Myc
Vector Length
4608bp
Vector Type
Phagemid vector
Vector Map

Background

Applications

Publications

Q & A

Customer Reviews

The pCDisplay-3M vector is a crucial phagemid vector. One significant application of the pCDisplay-3M vector lies in selecting and isolating specific proteins or peptides from libraries. The pCDisplay-3M phagemid vector has a considerable length of 4608 base pairs. The vector includes two fused tags - His6 and Myc. The His6 tag allows the convenient purification of the expressed proteins using nickel-chelating resins due to its high affinity for Ni²⁺ ions, while the Myc tag aids in detecting the expression of the recombinant protein through Western blot analysis or immunostaining, thereby enhancing the versatility and flexibility in downstream applications. One of the key attributes of the pCDisplay-3M vector is that it incorporates TG1 as its host cell. TG1 is a popular bacterial strain extensively applied in molecular cloning due to its high transformation efficiency. It provides a viable and robust environment for the propagation of the pCDisplay-3M vector, thereby allowing successful gene cloning. Notably, the pCDisplay-3M vector contains two promoters – M13 and ColE, both of which control the transcription of the target gene, therefore making the pCDisplay-3M vector a highly inducible system. The use of M13 and ColE essentially grants control over the gene expression, making this phagemid vector a valuable tool for studying gene regulation and function.
The pCDisplay-3M vector, a critical phagemid vector, possesses an assortment of uses in biotechnological and biological research. Its application primarily pertains to the field of molecular cloning, which plays a vital role in biotechnology to study and modify genes and proteins to create an organization of genetic material. One of the prominent applications of the pCDisplay-3M vector occurs in the bioengineering of proteins, where it is of fundamental importance and used for the expression of recombinant proteins. The vector allows for the construction and subsequent expression of varieties of fusion proteins within bacteria. This application aids in the production of proteins in large quantities for later use in numerous studies such as therapeutic medicine, enzymology, and immunology. The pCDisplay-3M vector's use is also notable in the field of gene therapy, genetically modifying a patient's cells to treat or cure disease. Here, the vector is employed to carry and deliver healthy copies of faulty genes into cells, helping to correct genetic disorders. The vector's ability to incorporate its genetic material into the host genome is crucial for the long-term efficacy of these gene therapies. The pCDisplay-3M vector is even used in functional genomics and proteomics studies, enabling comprehensive analysis to understand gene behavior and protein dynamics at an organism level. It plays a crucial role in proteomic research by allowing for the production of a broad range of proteins, aiding in identifying proteins' functions and interactions.
Customer Q&As
Is the phagemid vector of pCDisplay-3M applicable for direct periplasmic expression of the sdAb from selected clone?

A: Yes. The pCDisplay-3M can be used for direct periplasmic expression of the sdAb.

Do I need an import permit for a phage?

A: No, phages are not considered pathogenic to humans, animals, or plants.

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Customer Reviews
Efficient conversion

I've been using the pCDisplay-3M vector for a while now and it's definitely playing an important role in my research. The vector provides an efficient conversion in terms of recombinant protein expression.

French

08/09/2023

Great!

The pCDisplay-3M vector is an essential tool that has enabled successful achievements in our DNA cloning and expression works. Highly recommended for any lab engaging in advanced genetic manipulations.

United Kingdom

10/19/2023

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