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pCDisplay-m Vector

For research use only. Not intended for any clinical use.
Cat.No.
VPT4040
Description
pCDisplay-m is a mammalian expression vector designed to target recombinant proteins to the surface of mammalian cells. Displayed proteins can be analyzed for their ability to interact with known or putative ligands. Proteins of interest are targeted and anchored to the cell surface by cloning the gene of interest in frame with the vectors unique N-terminal secretion signal and the C-terminal transmembrane anchoring domain of platelet-derived growth factor receptor (PDGFR). In contrast to phage display vectors which operate exclusively in prokaryotic cells, the pCDisplay-m vector offers the advantage of having the displayed protein of interest processed in mammalian cells. Therefore, recombinant proteins of eukaryotic origin that are expressed from pCDisplay-m more closely resemble their native form. This may favor more accurate ligand binding interactions. In addition to the N-terminal cell surface targeting signal and C-terminal transmembrane anchoring domain.
Promoter
CMV
Resistance
Neomycin resistance marker for stable selection in mammalian cellsAmpicillin resistance gene for selection in E. coli
Selection
Neomycin resistance marker for stable selection in mammalian cellsAmpicillin resistance gene for selection in E. coli
Tag
c-Myc Epitope Tag,PDGFR Transmembrane Domain, HA Tag
Vector Length
5.3kb
Vector Type
phagemid vector
Vector Map

Background

Applications

Publications

Q & A

Customer Reviews

The pCDisplay-m vector is a well-crafted mammalian expression vector specifically designed to target recombinant proteins to the surface of mammalian cells. The significant advantage of this vector is the ability to analyze displayed proteins for their interaction capacity with established or potential ligands. The proteins of interest are purposefully targeted and secured to the cell surface via the cloning of the relevant gene concurrently with the vector's unique N-terminal secretion signal as well as the C-terminal transmembrane anchoring domain of the commonly known platelet-derived growth factor receptor (PDGFR). A distinctive feature of the pCDisplay-m vector, in comparison to phage display vectors which operate solely in prokaryotic cells, is the benefit of processing the displayed protein of interest within the mammalian cells. As a result, recombinant proteins of eukaryotic origin displayed from the pCDisplay-m are closer to their native form in terms of representation. This specificity and closeness in representation can potentially allow for more accurate binding interactions with ligands. In addition to the N-terminal cell surface targeting signal and C-terminal transmembrane anchoring domain, this vector also provides resistance markers.
pCDisplay-m Vector has been increasingly utilized in a promising approach to analyze protein-protein interactions in mammalian cells. The essential function of this vector is that it is formatted to target recombinant proteins towards the surface of mammalian cells. This particular application has opened new avenues to the understanding of biological functions within cells. pCDisplay-m exploits the potential of protein-ligand interactions, allowing functional analysis of displayed proteins corresponding to known or putative ligands. This approach enables the study of cellular processes broadly, in particular how the displayed proteins interact with other ligands and their subsequent effects in the cell. The process of new drug discovery and development greatly depends on the identification of potential biological targets. In this context, pCDisplay-m method provides valuable insights, since understanding the interactions between proteins and ligands can potentially identify new drug targets. Furthermore, pCDisplay-m Vector also appears to be an instrumental tool for the antibody development process. Recombinant proteins displayed on the surface of cells serve as antigens to elicit an immune response. Hence, the pCDisplay-m Vector effectively initiates this process leading to the formation of antibodies that can aid in the therapeutic interventions of various diseases.
Customer Q&As
What are the applications of phagemid vectors?

A: Phagemid vectors are commonly used in techniques such as phage display, where a protein or peptide library is displayed on the surface of the assembled phage particles. This allows for the screening of large numbers of proteins or peptides for specific interactions with target molecules.

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Customer Reviews
Excellent product!

Excellent product! The pCDisplay-m vector is a highly specialized tool that has enhanced the precision of my work in studying protein interactions.

United Kingdom

01/16/2024

Great tool

I have been using the pCDisplay-m vector in my experiments, and it has significantly streamlined my research process. The ability to analyze displayed proteins for their interaction potentials has opened up new avenues in my study. Great tool for mammalian expression studies!

United Kingdom

06/02/2020

Very satisfied

The pCDisplay-m vector has undoubtedly soared my protein studies to new heights. Targeting recombinant proteins to the surface of mammalian cells had never been this efficient.

French

04/17/2023

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