fADL-display Vector

The fADL-display vector is a type 3 phage display vector. It is distinct due to its cloning site situated on the N-terminal side of the full-length gene III protein. Moreover, it uses the PelB leader peptide to drive the secretion of the fusion protein into the periplasm, which is an innovative alternative to utilizing the wild-type gene III protein leader sequence. fADL-1 originates from the fd-kan phage vector, which shares substantial similarities with the fd-tet vector. The primary difference between the two lies in the swapping out of the tetracycline resistance genes originally derived from Tn10 and their replacement with the kanamycin resistance determinant accumulated from Tn903. One major attribute of the fADL-display vector is its display capability, being generally multivalent with limited polyphage production. This trait holds even when processing sizeable polypeptides, such as scFvs. Owing to this attribute, it is advisable to deploy this format when dealing with peptide libraries, primarily because of the increased avidity brought about by the vector. The genetic makeup of fADL-display vector includes a kanamycin resistance (Kan) trait, that makes it resistant to kanamycin, enabling it easier for researchers to distinguish transformed from non-transformed cells.