mCherry/Luciferase reporter cell line - A20

The A20 cell line, established in 1971 by Hamburger et al., originates from a spontaneous reticulum cell sarcoma in a BALB/cAnN mouse. Initially utilized as a tool to study lymphoma pathogenesis, A20 cells exhibit characteristics typical of B lymphocytes, including surface immunoglobulins and the ability to undergo class switching. Over time, researchers have extensively employed A20 cells to elucidate various signaling pathways and immune responses, owing to their amenability to genetic manipulation and stable propagation.

Subsequent advancements in molecular biology techniques facilitated the development of reporter cell lines incorporating fluorescent or luminescent markers to monitor gene expression and cellular activities. The fusion of A20 cells with reporter genes such as mCherry and Luciferase has revolutionized the study of molecular mechanisms underlying immune responses, tumorigenesis, and therapeutic interventions. The mCherry reporter, a red fluorescent protein derived from Discosoma sp., enables visual tracking of A20 cells in vivo and in vitro with exceptional brightness and photostability. In parallel, the incorporation of Luciferase, a light-emitting enzyme from fireflies, into A20 cells provides a sensitive and quantitative means to assess transcriptional activity and cellular viability.