Cat.No. : CSC-RR0460 Host Cell: MM1.S
| Cat. No. | CSC-RR0460 |
| Description | MM1.S-Luc cell line is engineered to stably overexpress luciferase under a constitutive promoter. It is an ideal positive control for use in bioluminescence assay. |
| Host Cell | MM1.S |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A haematologic cancer, multiple myeloma (MM) causes great morbidity using the growth of aberrant plasma B-cells. Targeting treatments include the monoclonal antibody daratumumab, which binds to CD38, a protein overexpressed in MM cells, and is under active research. This work assessed utilizing the MM1.S-luciferase cell line in vitro and in vivo the imaging potential of [89Zr] Zr-DFO-daratumumab in MM models. Findings showed high specificity and affinity for CD38, indicating the potential of this bioconjugate for imaging and therapeutic applications.
![Figure 1 illustrates the validation of CD38 expression in human MM1.S myeloma cells via flow cytometry, the saturation binding curve for [89Zr]Zr-DFO-daratumumab, and the percentage of cell uptake of the conjugate at 37°C with and without a blocking dose of cold daratumumab. (doi: 10.2967/jnumed.117.196063)](/upload/image/luciferase-stable-cell-line-mm1-s-1.jpg)
Figure 1. The researchers employed flow cytometry to confirm CD38 expression in MM1.S cells and conducted saturation binding assays to evaluate [89Zr]Zr-DFO-daratumumab uptake. (Ghai A, et al., 2018)
Creative Biogene's Luciferase Stable Cell Line-MM1.S is designed for similar research directions, offering a robust platform for studying MM and testing novel therapeutics. This cell line provides researchers with a reliable tool for evaluating drug efficacy and understanding tumor biology in a controlled environment. The integration of luciferase enables sensitive detection of cell proliferation and tumor burden in various experimental setups, facilitating advancements in targeted therapies and imaging strategies for MM.
A: To maintain consistent luciferase expression in the Luciferase Stable Cell Line-MM1.S, it's crucial to grow the cells in a recommended medium supplemented with the appropriate selection antibiotic, as per the supplier's instructions. Cells should be cultured at 37°C in a humidified atmosphere containing 5% CO2, avoiding over-confluency. Periodic testing for luciferase activity is recommended to monitor the stability of expression. Additionally, freezing multiple early passage stocks can help preserve the cell line with stable luciferase expression.
A: Quantifying luciferase activity in the Luciferase Stable Cell Line-MM1.S requires the use of a luminometer and a luciferase assay reagent. After collecting the cells, they should be lysed with a compatible lysis buffer, and the cell lysates are then mixed with the luciferase assay reagent. The light emitted is measured using the luminometer. For bioluminescent imaging, it's important to calibrate the imaging system to ensure accurate quantification, taking into account factors such as exposure time, distance, and sensitivity settings.
A: The Luciferase Stable Cell Line-MM1.S is generally considered to be at Biosafety Level 1 (BSL-1), assuming no hazardous genes have been introduced into the cell line. Standard safety practices for handling cell cultures should be followed, including wearing gloves, lab coats, and eye protection. All materials coming into contact with the cell cultures should be autoclaved or treated with appropriate disinfectants before disposal. Any specific safety data sheets (SDS) provided by the manufacturer should also be consulted for detailed handling and disposal guidelines.
A: Yes, the Luciferase Stable Cell Line-MM1.S can be used alongside fluorescence-based reporters for multiplex assays. It is important to select fluorescent reporters that emit light at wavelengths distinctly different from the bioluminescence emitted by luciferase to avoid spectral overlap. Additionally, the assay protocol should be optimized to ensure that the luminescence and fluorescence measurements do not interfere with each other, which might involve sequential measurements or the use of filters. Pilot experiments to validate the multiplexing approach and optimize detection parameters are recommended.
A: Ensuring long-term genomic stability of the Luciferase Stable Cell Line-MM1.S involves several key strategies. Firstly, minimizing the number of passages and using low passage cells for critical experiments can reduce the risk of genomic drift. Secondly, cells should be regularly monitored for phenotypic and genotypic changes, especially when exposed to experimental conditions that might induce stress or genomic alterations. Implementing cryopreservation of early passage cells and periodic validation of luciferase expression and cell line identity through techniques such as short tandem repeat (STR) profiling are also advisable to maintain genomic integrity.
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With luciferase expression, the Luciferase Stable Cell Line-MM1.S provides high sensitivity for detecting promoter activity, crucial for studying gene regulation and signaling pathways.
This cell line is homogeneous, offering a uniform response to experimental conditions. The Luciferase Stable Cell Line-MM1.S ensures consistent behavior in assays, which is critical for high-quality, reproducible data.
The luciferase assay is straightforward and can be easily integrated into the workflow, making the Luciferase Stable Cell Line-MM1.S user-friendly for routine lab applications.
Luciferase expression allows for the in vivo tracking of the Luciferase Stable Cell Line-MM1.S, essential for studying tumor growth and metastasis in live animal models.
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