Cat.No. : CSC-SC015517-1 Host Cell: HEK293
| Cat. No. | CSC-SC015517-1 |
| Description | This cell line is engineered to stably express human trace amine associated receptor 1 (TAAR1) in HEK293 cells. |
| Gene | TAAR1 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Trace amine-associated receptor 1 (TAAR1) activation is essential for weight regulation and glucose homeostasis in the endocrine pancreas. Obese people with poor glucose metabolism have been studied for TAAR1 gene variations. The researchers screened 314 patients with obesity and compromised insulin secretion, uncovering several single nucleotide missense variants, including p.Arg23Cys, p.Ser49Leu, and p.I171Leu. These variants were assessed for their effect on TAAR1 cell surface expression and signaling capabilities. Notably, the p.Arg23Cys variant was linked to a complete loss of insulin production, while p.Ser49Leu and p.I171Leu showed varying degrees of function. The findings suggest that these naturally occurring variants could significantly impact weight regulation and glucose balance.
Figure 1. The researchers expressed wild-type TAAR1 and the three mutant variants in HEK293 cells to assess T1AM-induced cAMP accumulation. They measured the signaling properties to evaluate how specific mutations affect receptor function, revealing significant reductions in the R23C variant's activity. (Mühlhaus J, et al., 2017)
For studies of this nature, our Human TAAR1 Stable Cell Line - HEK293 is ideally suited. This cell line allows for precise exploration of GPCR structure-function relationships and offers a robust platform for functional assays, including cAMP accumulation and cell surface expression assessments.
A: The TAAR1 gene encodes for the trace amine-associated receptor 1, a G protein-coupled receptor. TAAR1 is involved in the detection of trace amines, which are low concentrations of biogenic amines with roles in neurotransmission, hormone regulation, and immune response.
A: The TAAR1 gene product plays a role in neurotransmission by responding to trace amines, such as tyramine and β-phenylethylamine. Activation of TAAR1 can modulate the release of neurotransmitters, including dopamine and norepinephrine, influencing mood, reward, and arousal.
A: Polymorphisms in the TAAR1 gene have been associated with various conditions, including schizophrenia and bipolar disorder. Altered TAAR1 function may contribute to imbalances in neurotransmitter levels, potentially affecting brain development and mental health.
A: The expression of the TAAR1 gene is regulated by various factors, including transcription factors and signaling pathways. TAAR1 expression can be influenced by environmental factors, such as stress and drug exposure, as well as by internal factors like hormone levels and neuronal activity.
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The supplier of the Human TAAR1 Stable Cell Line - HEK293 is committed to continuous innovation and upgrades, incorporating the latest advancements in gene editing technologies. This ensures that the cell line remains at the forefront of scientific research, meeting the evolving needs of the research community.
The Human TAAR1 Stable Cell Line - HEK293 benefits from an active user community and forum, where researchers can share experiences, troubleshoot issues, and stay updated on the latest advancements in using this cell line. This community provides a valuable resource for researchers to learn from each other and optimize their experimental procedures.
The Human TAAR1 Stable Cell Line - HEK293 exhibits a high degree of knockout efficiency, successfully suppressing the TAAR1 gene. This level of efficiency is essential for precisely investigating the function of TAAR1 in numerous biological pathways and its potential contributions to the onset of diseases.
The cell line preserves superior genetic stability, ensuring that the knockout phenotype remains steady across many passages. This consistency is vital for obtaining trustworthy experimental outcomes and for the sustained viability of the cell line, which is particularly important for extended research projects.
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