Human SRD5A2 Stable Cell Line - HEK293

Name: Human SRD5A2 Stable Cell Line - HEK293
SKU: CSC-SC015139-1
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC015139-1

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

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Gene Information

Cat. No.	CSC-SC015139-1
Description	This cell line is engineered to stably express human steroid 5 alpha-reductase 2 (SRD5A2) in HEK293 cells.
Target Gene	SRD5A2
Gene Species	Homo sapiens (Human)
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	SRD5A2 steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2) [ Homo sapiens ]
Gene Symbol	SRD5A2
Gene Description	steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2)
GeneID	6716
Uni ProtID	P31213
mRNA Refseq	NM_000348.3
Protein Refseq	NP_000339.2
Chromosome Location	2p23
Function	3-oxo-5-alpha-steroid 4-dehydrogenase activity; progesterone 5-alpha-reductase activity; sterol 5-alpha reductase activity;
Pathway	Androgen biosynthesis, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of lipids and lipoproteins, organism-specific biosystem; Metabolism of steroid hormones and vitamins A and D, organism-specific biosystem; Prostate cancer, organism-specific biosystem; Prostate cancer, conserved biosystem; Steroid hormone biosynthesis, organism-specific biosystem;
MIM	607306

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Background

Case Study

Applications

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The Human SRD5A2 (Steroid 5-Alpha-Reductase Type 2) gene encodes an enzyme that catalyzes the conversion of testosterone to dihydrotestosterone, a more potent androgen. SRD5A2 plays a critical role in the development of male reproductive tissues and secondary sexual characteristics. In addition, SRD5A2 is involved in the regulation of androgen levels in the brain, which can impact cognitive function and mood.

The HEK293 cell line has been engineered to stably express the Human SRD5A2 gene. This stable cell line provides a valuable tool for studying the role of SRD5A2 in androgen metabolism and its implications in various physiological and pathological conditions, including male reproductive health, prostate cancer, and cognitive disorders. Researchers can use the HEK293 SRD5A2 stable cell line to investigate the enzyme's activity, its regulation, and its impact on cellular processes. This cell line also serves as a platform for drug metabolism studies and the development of treatments for conditions related to SRD5A2 activity.

The enzyme steroid 5-α reductase (5AR) converts steroids into 5-α reduced metabolites, such as 5-α dihydrotestosterone (DHT) from testosterone. Researchers developed a cell-based assay to evaluate the inhibition of 5AR, crucial for DHT production. IC50 values for inhibitors finasteride and dutasteride were determined using human and fish cell lines via transient overexpression. Fish cell lines showed a lower IC50 for dutasteride than human cell lines, suggesting species-specific responses. This sensitive assay, employing chemical derivatization and LC-MS/MS, exhibits high reproducibility and is suitable for screening endocrine-disrupting chemicals (EDCs). Future studies will explore 5AR inhibition by EDCs across species to elucidate their impact on endocrine pathways.

Figure 1 depicts DHT levels measured in non-vector- and SRD5A1-HEK293 cells and SRD5A2-HEK293 cells following testosterone addition. (doi: 10.3390/molecules26040893) Figure 1. DHT levels were measured in non-vector-, SRD5A1-HEK293, and SRD5A2-HEK293 cells following testosterone addition. KM values reflecting enzyme affinity were calculated, followed by inhibition assays with finasteride and dutasteride to determine IC50 values (Kim D, et al., 2021).

Human SRD5A2 Stable Cell Line - HEK293 is engineered to stably express steroid 5 alpha-reductase 2, an enzyme that plays a pivotal role in the conversion of testosterone into the more potent dihydrotestosterone (DHT). This conversion is crucial for the development and maintenance of male secondary sexual characteristics and also plays a role in the pathophysiology of several diseases.

(1)Drug Development for Prostate Diseases

This cell line is instrumental for screening and evaluating novel inhibitors of SRD5A2 that could be used for the treatment of benign prostatic hyperplasia (BPH) and prostate cancer. By inhibiting the conversion of testosterone to DHT, the progression of prostate enlargement and malignancy can potentially be controlled or slowed.

(2)Research on Androgenic Alopecia

The Human SRD5A2 Stable Cell Line - HEK293 can also be used in the study of androgenic alopecia, commonly known as male pattern baldness. Researchers can test the efficacy of topical treatments aimed at reducing DHT levels in the scalp and thus slowing or reversing hair loss.

(3)Understanding Hormonal Regulation and Sexual Differentiation

The cell line provides a consistent model to explore the role of androgens in sexual differentiation and development. It allows for the in vitro study of genetic disorders of sexual development, such as 5-alpha-reductase deficiency, contributing to a better understanding of this enzyme's function in physiological and pathological conditions.

Customer Q&As

What is the effect of the Human SRD5A2 Stable Cell Line - HEK293 in studying androgen metabolism?

A: The role of the Human SRD5A2 Stable Cell Line - HEK293 in studying androgen metabolism is to provide an experimental platform to investigate how the SRD5A2 enzyme catalyzes the conversion of testosterone to dihydrotestosterone, a process crucial for the male reproductive system and prostate health. This cell line helps elucidate the role of the SRD5A2 gene in androgen-related diseases, such as prostate cancer and benign prostatic hyperplasia.

What is the role of the Human SRD5A2 Stable Cell Line - HEK293 in studying androgen receptor signaling?

A: The role of the Human SRD5A2 Stable Cell Line - HEK293 in studying androgen receptor signaling is to investigate how the stable expression of the SRD5A2 enzyme affects the activation and signaling of androgen receptors. This cell line helps reveal the role of SRD5A2 in regulating androgen signals and cellular functions, which is crucial for understanding the molecular mechanisms of androgen-related diseases.

What is the role of the Human SRD5A2 Stable Cell Line - HEK293 in identifying potential therapeutic targets for androgen-related diseases?

A: The role of the Human SRD5A2 Stable Cell Line - HEK293 in identifying potential therapeutic targets for androgen-related diseases is to provide an experimental model for evaluating the impact of SRD5A2 enzyme inhibitors on disease progression. This cell line aids in discovering new therapeutic strategies, especially in the development of treatments for prostate cancer and benign prostatic hyperplasia.

What is the role of the Human SRD5A2 Stable Cell Line - HEK293 in studying the effects of androgens on cell proliferation?

A: The role of the Human SRD5A2 Stable Cell Line - HEK293 in studying the effects of androgens on cell proliferation is to simulate the role of androgens in cell growth and differentiation by stably expressing the SRD5A2 enzyme. This cell line helps understand the function of SRD5A2 in regulating cell growth and maintaining tissue homeostasis, which is significant for developing treatments that inhibit excessive cell proliferation.

How is the stable expression of the SRD5A2 gene achieved in the Human SRD5A2 Stable Cell Line - HEK293?

A: The stable expression of the SRD5A2 gene in the Human SRD5A2 Stable Cell Line - HEK293 is achieved by integrating the SRD5A2 gene into the genome of HEK293 cells, using selective markers and screening strategies. This typically involves the use of viral vectors, such as retroviruses or lentiviruses, and antibiotic resistance genes as selection markers, thereby creating a cellular model that continuously expresses the SRD5A2 protein.

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Customer Reviews

Drug Development

Provides a platform for developing therapeutic drugs targeting SRD5A2-related diseases.

Canada

2020-08-20

Disease Mechanism Exploration

Contributes to understanding the association between SRD5A2 gene mutations and disease development.

United Kingdom

2023-04-25

Hormone Metabolism Research

The Human SRD5A2 Stable Cell Line - HEK293 aids in studying the role of the SRD5A2 gene in hormone metabolism.

United States

2020-03-26

Cell Function Research

Enhances knowledge of related physiological processes by studying the function of SRD5A2 within cells.

United States

2020-07-21

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Human SRD5A2 Stable Cell Line - HEK293

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Background

Case Study

Applications

Q & A

Customer Reviews

What is the effect of the Human SRD5A2 Stable Cell Line - HEK293 in studying androgen metabolism?

What is the role of the Human SRD5A2 Stable Cell Line - HEK293 in studying androgen receptor signaling?

What is the role of the Human SRD5A2 Stable Cell Line - HEK293 in identifying potential therapeutic targets for androgen-related diseases?

What is the role of the Human SRD5A2 Stable Cell Line - HEK293 in studying the effects of androgens on cell proliferation?

How is the stable expression of the SRD5A2 gene achieved in the Human SRD5A2 Stable Cell Line - HEK293?

Drug Development

Disease Mechanism Exploration

Hormone Metabolism Research

Cell Function Research

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