Cat.No. : CSC-RO0685 Host Cell: HEK293
| Cat. No. | CSC-RO0685 |
| Description | This cell line is engineered to stably human spectrin beta, non-erythrocytic 1 (SPTBN1). |
| Gene | SPTBN1 |
| Host Cell | HEK293 |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
SPTBN1 exerts anticancer effects in a variety of tumors and is involved in chemoresistance in epithelial ovarian cancer (EOC). Here, researchers report that lower SPTBN1 expression was significantly associated with advanced EOC stage and shorter progression-free survival. SPTBN1 expression was also higher in less aggressive EOC cell lines. Furthermore, SPTBN1 reduced the migration capacity of EOC cells A2780 and HO8910 and inhibited the growth of EOC cells in vitro and tumor xenografts in vivo. SPTBN1 inhibition increased the epithelial-mesenchymal transition marker Vimentin, while reducing the expression of E-cadherin. SOCS3 overexpression or JAK2 inhibition reduced the proliferation and migration of EOC cells, as well as the expression of p-JAK2, p-STAT3, and Vimentin, while SPTBN1 downregulation enhanced these expressions, while the expression of E-cadherin was reversed. It was also demonstrated in mouse embryonic fibroblasts (MEFs) that loss of SPTBN1 activated the JAK/STAT3 signaling pathway while inhibiting SOCS3. These findings suggest that SPTBN1 inhibits the progression of epithelial ovarian cancer through SOCS3-mediated blockade of the JAK/STAT3 signaling pathway.
The results showed that SPTBN1 overexpression inhibited HO8910 cell migration (Figure 1A), while SPTBN1 inhibition promoted A2780 cell migration (Figure 1B).
Figure 1. Cell migration assessment. Overexpression of SPTBN1 inhibits the migration of HO8910 cells (A), whereas downregulation of SPTBN1 promotes the migration of A2780 cells (B). (Chen M, et al., 2020)
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
Producing enough protein for biochemical analysis used to be a major bottleneck in our research. The Human SPTBN1 Stable Cell Line now provides us with a robust and high-yield system that simplifies our workflow and allows us to focus more on the analytical part rather than the production phase.
We have been using Human SPTBN1 Stable Cell Line - HEK293 for compound screening in our drug discovery pipeline. The robustness and predictability of this cell line make it an ideal model for high-throughput screening, allowing us to identify potential drug candidates efficiently and with greater confidence.
The Human SPTBN1 Stable Cell Line consistently provides reliable results in our signaling experiments. This greatly increases our confidence in our findings.
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.
Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
Copyright © Creative Biogene. All rights reserved.
