Cat.No. : CSC-RO0688 Host Cell: HEK293T
Size: >1x10^6 frozen cells/vial, 1 mL Stability: Stable in culture over a minimum of 10 passages
| Cat. No. | CSC-RO0688 |
| Description | This cell line is engineered to stably overexpress human receptor tyrosine kinase like orphan receptor 2 (ROR2). |
| Target Gene | ROR2 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Stable in culture over a minimum of 10 passages |
| Application | Drug screening and biological assays |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Size | >1x10^6 frozen cells/vial, 1 mL |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Given the current treatment landscape, there is an urgent need to identify new disease markers and innovative therapeutic strategies for epithelial ovarian cancer (EOC), which stands as the deadliest cancer among female genital tumors. Researchers aimed to elucidate the role of ROR2 in high-grade serous ovarian carcinoma (HGSOC) cells and uncover the underlying mechanism. Immunohistochemistry and western blot assays were employed to assess protein expression. ROR2 overexpression models were created using adenovirus and Lentivirus in vitro and in vivo, respectively. Functional assays including MTT, colony formation, and transwell assays were utilized to evaluate cell proliferation, invasion, and migration. Flow cytometry was employed to measure apoptosis rates. Whole transcriptome analysis was conducted to identify differentially expressed genes.
Figure 1. The effect of ROR2 overexpression on HGSCO cell behavior was investigated by researchers. ROR2 expression at protein and mRNA levels in various cell lines was assessed using Western blot and PCR assays. Proliferation and clonogenic potential after ROR2 overexpression were evaluated through MTT and colony formation assays. Results suggest inhibition of cell proliferation and colony formation in HGSCO cells by ROR2. (Li R, et al., 2019)
A: HEK293T cells were chosen for establishing the stable cell line expressing human ROR2 due to their robust transfection efficiency and suitability for protein expression studies. HEK293T cells are widely used for overexpression experiments and offer a suitable cellular environment for studying ROR2 signaling pathways.
A: The stability and expression level of human ROR2 in the HEK293T stable cell line was assured and sustained through stable transfection, followed by validation of expression using techniques such as western blot analysis and immunofluorescence microscopy. Continuous culture under appropriate conditions ensured stable expression.
A: Functional characterization of human ROR2 in the HEK293T stable cell line involved investigating its role in cell migration, differentiation, and signaling pathways. This included evaluating downstream signaling events such as Wnt signaling pathway activation and assessing effects on cellular behaviors such as migration and proliferation.
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Impressive reliability. This cell line surpasses expectations, serving as a robust platform for studying ROR2-targeted therapies and potential interventions for ROR2-related disorders. Its stable expression simplifies experimental workflows, facilitating efficient data collection and analysis, and accelerating discoveries in developmental biology and cancer biology.
The Human ROR2 Stable Cell Line has significantly enhanced my research capabilities, offering valuable insights into ROR2-mediated signaling pathways and potential therapeutic strategies for ROR2-associated diseases.
The Human ROR2 Stable Cell Line in HEK293T cells ensures consistent expression of ROR2, enabling reliable results in my research on developmental biology and cancer. I can investigate Wnt signaling pathways and ROR2-mediated cellular processes with confidence, advancing our understanding of embryogenesis and tumorigenesis.
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