Cat.No. : CSC-DC013462-1
Host Cell: MCF7 Validation: Real-Time RCR
| Cat. No. | CSC-DC013462-1 |
| Description | This cell line is engineered to stably overexpress shRNA targeting human ROCK2. Expression of human ROCK2 gene is stably knocked down in this cell line. |
| Gene | ROCK2 |
| Host Cell | MCF7 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | (1) Studying gene functions (2) Studying gene interactions and signaling pathways (3) Target validation and drug discovery (4) Designing diseases models |
| Size Form | >1 × 10^6 cells / vial |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
The Rho kinases, ROCK1 and ROCK2, facilitate actomyosin cytoskeletal reorganization by acting as a downstream effect of the small GTPase RhoA. The roles of Rho kinases (ROCK1 and ROCK2) in actomyosin cytoskeletal reorganization were studied by the researchers using knockdown cell lines. To selectively reduce ROCK1 and ROCK2, they used certain siRNA. ROCK1 knockdown was found to cause disruptions in cadherin organization at the epithelial zonula adherens (ZA), which in turn resulted in the loss of nonmuscle myosin IIA (NMIIA) and F-actin. ROCK2 knockdown, however, had no discernible impact. Furthermore, ROCK1—but not ROCK2—was essential for preserving junctional tension, stopping intraepithelial cell migration, and stabilizing GTP-RhoA at the ZA. This suggests that at the epithelial ZA, ROCK1 is necessary for both contractile tension and junctional integrity.
Figure 1. In order to investigate ROCK2's function in biological processes, the researchers used ROCK2 knockdown in MCF-7 cells. They used line-scan analysis to observe the location of E-cadherin and F-actin, and they used nanoablation to examine junctional tension and epithelial mobility. Understanding the role of ROCK2 in epithelial cell dynamics has been aided by confocal imaging, which has shed light on how the protein moves and arranges cell junctions. (Priya R, et al., 2017)
A: By knocking down ROCK2 expression, researchers can study the effects on breast cancer cell motility, invasion, and metastasis. This can help identify the molecular mechanisms underlying these processes and potential therapeutic targets for metastatic breast cancer.
A: The cell line allows researchers to explore how ROCK2 influences signaling pathways involved in cell proliferation, survival, and epithelial-mesenchymal transition (EMT) in breast cancer. This can lead to a better understanding of the molecular basis of breast cancer progression and the development of targeted therapies.
A: By examining the effects of ROCK2 knockdown on the response of breast cancer cells to chemotherapeutic agents, researchers can identify potential mechanisms of drug resistance and develop strategies to overcome these challenges in cancer treatment.
A: The cell line can be used to study the interactions between ROCK2 and other signaling proteins, such as RhoA, in the context of breast cancer. This can reveal how these molecules coordinate to regulate cellular processes and contribute to cancer development.
A: By understanding the role of ROCK2 in breast cancer cell behavior, this cell line can help identify biomarkers that predict patient response to ROCK2-targeted therapies. This can inform the development of personalized treatment strategies based on the molecular profile of individual tumors.
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The Human ROCK2 Knockdown Cell Line - MCF7 effectively silences the ROCK2 gene, reducing its expression significantly. This trait is essential for studies aimed at understanding the role of ROCK2 in cellular processes like migration and proliferation, which are crucial in cancer research and therapy development.
This cell line facilitates enhanced studies on signal transduction pathways that involve ROCK2, a key player in cellular behavior and pathology. We can use the Human ROCK2 Knockdown Cell Line - MCF7 to dissect the signaling pathways and mechanisms where ROCK2 has a regulatory role, providing insights into tumor suppression and other cellular functions.
As MCF7 is a breast cancer cell line, the Human ROCK2 Knockdown Cell Line - MCF7 is particularly suited for oncological studies. It allows us to directly assess the impact of ROCK2 inhibition on cancer cell behavior, contributing to targeted therapy development and a deeper understanding of cancer biology.
This cell line is provided in a condition that is immediately usable for experiments, reducing the preparation time significantly. The Human ROCK2 Knockdown Cell Line - MCF7 being ready-to-use allows for a swift transition from setup to actual experimentation, facilitating a smoother research process.
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