Cat.No. : CSC-SC010841-1 Host Cell: HEK293
| Cat. No. | CSC-SC010841-1 |
| Description | This cell line is engineered to stably express human oxidized low density lipoprotein receptor 1 (OLR1) in HEK293 cells. |
| Gene | OLR1 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
The function of the lectin-like ox-LDL receptor 1 (OLR1) in carcinogenesis was studied by the researchers. The Olr1 knockout (KO) and wild-type (WT) mice were used to examine patterns of gene expression associated with cellular transformation. Their investigations on breast cancer cells (HCC1143) and normal mammary epithelial cells (MCF10A) included looking at gene expression, adhesion, migration, and transendothelial migration. 26 out of 238 genes, several of which have NF-κB binding sites in their promoters, were shown to be inhibited in Olr1 KO mice. Subsequent examination revealed widespread suppression of NF-κB target genes, affecting defensive and immunological responses, wound healing, apoptosis, and proliferation. Furthermore, de novo lipogenesis gene suppression was observed in Olr1 KO mice. When comparing cells from MCF10A with HCC1143, the latter showed increased expression of OLR1. By upregulating NF-κB and its target pro-oncogenes, forced overexpression of OLR1 in both cell lines improved cell migration and inhibited apoptosis.
Figure 1. By transfecting HCC1143 cells with either an OLR1 expression vector or an empty plasmid, the researchers evaluated the effects of OLR1 overexpression. They measured cell migration using a wound-healing assay. OLR1 overexpression was verified by qPCR. Compared to controls, cells exhibiting increased OLR1 levels showed faster wound closure (p<0.001). On the other hand, there was no discernible variation in the adhesion and transendothelial migration of cancer cells transfected with OLR1. Notably, baseline adhesion and transendothelial migration of non-transfected HCC1143 cells were reduced when OLR1 was inhibited or neutralized in endothelial cells. (Khaidakov M, et al., 2011)
A: The stability of this Human OLR1 Stable Cell Line-HEK293 cell line was verified through long-term culture and continuous monitoring of gene expression levels. Specific steps include antibiotic screening to ensure the survival of cells carrying the target gene, and RTPCR and Western Blotting to detect the stable expression of the OLR1 gene and protein. In addition, cellular functional experiments, such as binding or endocytosis assays, can further verify the sustained activity of OLR1 function. These steps combined ensure the stability of the cell line in long-term culture.
A: This cell line is mainly suitable for studying the biological functions of OLR1 in cells and its role in diseases such as atherosclerosis. Specific applications include studying OLR1-mediated low-density lipoprotein (LDL) receptor pathways, exploring the role of OLR1 in inflammatory responses, and evaluating the effectiveness of potential drugs targeting OLR1. Due to the ease of handling of the HEK293 cell line, this stable cell line is also ideal for high-throughput drug screening and functional validation.
A: Yes, this cell line undergoes strict quality control. Specific indicators include: cell morphology testing to ensure that cells have normal shape under the microscope; cell proliferation rate testing to evaluate cell health; mycoplasma contamination testing to ensure that cells are free of mycoplasma contamination; gene and protein expression testing to confirm the OLR1 gene and protein stable expression. In addition, cell cryopreservation and recovery experiments ensure that cells can recover their properties and functions after cryopreservation.
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The cell quality is really good. HEK293 cells grow stably during the culture process and their gene expression is also very consistent. Very suitable for long-term experimental use.
The HEK293 cell line of this platform is easy to use, the cells grow rapidly, the gene expression is stable, and there are no problems. Recommended to friends who need to do gene expression research.
The performance of this merchant's cell line is very reliable. HEK293 cells have shown excellent stability and fast growth rate in experiments, making them suitable for various biological studies.
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