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Cat.No. : CSC-RO0866 Host Cell: A549
| Cat. No. | CSC-RO0866 |
| Description | This cell line is engineered to stably overexpress human MELTF (also named as CD228). |
| Gene | MELTF |
| Gene Species | Homo sapiens (Human) |
| Host Cell | A549 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The impact of MELTF gene expression can be assessed by correlating the levels of expression in the A549 cell line with tumor growth rate, invasiveness, and drug sensitivity. Quantitative measurement of changes in MELTF expression can be achieved through RNA sequencing and proteomic analysis.
A: While the A549 cell line can mimic certain aspects of lung cancer, it may not fully replicate the complexity of the tumor microenvironment in the human body, such as immune responses or angiogenesis. Additionally, genetic drift in long-term cultured cell lines can reduce the reproducibility of results.
A: The effect of new drugs on the A549 cell line can be assessed using cell viability assays, apoptosis analysis, and cell cycle analysis. Monitoring changes in MELTF gene expression can provide insights into the mechanisms of drug action. High-throughput screening and real-time cellular imaging can enhance the accuracy and efficiency of the experiments.
A: The A549 cell line can be used to study the metabolic characteristics of lung cancer cells, especially against the backdrop of altered MELTF gene expression. Understanding the metabolic regulation mechanisms in lung cancer can be improved by measuring changes in metabolites and metabolic pathway activities.
A: The A549 cell line can be used as a host cell model to study the process of viral infection, particularly in the context of pulmonary pathogens. Researching how viruses alter MELTF gene expression and how these changes affect cellular responses can reveal the interactions between viruses and host cells.
A: The MELTF gene may play a significant role in regulating cell growth and death. Studying the effects of overexpression or silencing of the MELTF gene on aging and apoptosis in A549 cells can provide deeper insights into the role of this gene in determining cell fate.
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