Human MELTF Stable Cell Line - A549

Name: Human MELTF Stable Cell Line - A549
SKU: CSC-RO0866
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0866

Host Cell : A549 Size : >1x10⁶ frozen cells/vial

Inquire for Price

Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No.	CSC-RO0866
Description	This cell line is engineered to stably overexpress human MELTF (also named as CD228).
Target Gene	MELTF
Gene Species	Homo sapiens (Human)
Host Cell	A549
Host Cell Species	Homo sapiens (Human)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

How can the impact of changes in MELTF gene expression on tumor growth be assessed in studies using the Human MELTF Stable Cell Line - A549?

A: The impact of MELTF gene expression can be assessed by correlating the levels of expression in the A549 cell line with tumor growth rate, invasiveness, and drug sensitivity. Quantitative measurement of changes in MELTF expression can be achieved through RNA sequencing and proteomic analysis.

What are the limitations of using the Human MELTF Stable Cell Line - A549 in simulating the lung cancer microenvironment?

A: While the A549 cell line can mimic certain aspects of lung cancer, it may not fully replicate the complexity of the tumor microenvironment in the human body, such as immune responses or angiogenesis. Additionally, genetic drift in long-term cultured cell lines can reduce the reproducibility of results.

How can the response of the Human MELTF Stable Cell Line - A549 to new drugs be accurately assessed in drug screening experiments?

A: The effect of new drugs on the A549 cell line can be assessed using cell viability assays, apoptosis analysis, and cell cycle analysis. Monitoring changes in MELTF gene expression can provide insights into the mechanisms of drug action. High-throughput screening and real-time cellular imaging can enhance the accuracy and efficiency of the experiments.

What role does the Human MELTF Stable Cell Line - A549 play in studying the metabolic regulation of lung cancer?

A: The A549 cell line can be used to study the metabolic characteristics of lung cancer cells, especially against the backdrop of altered MELTF gene expression. Understanding the metabolic regulation mechanisms in lung cancer can be improved by measuring changes in metabolites and metabolic pathway activities.

How can the Human MELTF Stable Cell Line - A549 help in understanding the interactions between viruses and host cells in viral infection models?

A: The A549 cell line can be used as a host cell model to study the process of viral infection, particularly in the context of pulmonary pathogens. Researching how viruses alter MELTF gene expression and how these changes affect cellular responses can reveal the interactions between viruses and host cells.

What role does the MELTF gene play in studies of cell aging and apoptosis using the Human MELTF Stable Cell Line - A549?

A: The MELTF gene may play a significant role in regulating cell growth and death. Studying the effects of overexpression or silencing of the MELTF gene on aging and apoptosis in A549 cells can provide deeper insights into the role of this gene in determining cell fate.

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Question *

Name

Country *

Email *

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Product Name *

Catalog Name *

Rating

Needs improvement

Satisfaction

General satisfaction

Very satisfaction

Product Review Title *

Summary *