Human HHLA2 Stable Cell Line - HEK293T

Name: Human HHLA2 Stable Cell Line - HEK293T
SKU: CSC-RO0524
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0524

Host Cell : HEK293T Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0524
Description	This cell line is derived from HEK293T and is engineered to stably overexpress Human HHLA2.
Target Gene	HHLA2
Gene Species	Homo sapiens (Human)
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	HHLA2 HERV-H LTR-associating 2 [ Homo sapiens ]
Gene Symbol	HHLA2
Synonyms	B7H7
GeneID	11148
Uni ProtID	Q9UM44
mRNA Refseq	NM_007072.2
Protein Refseq	NP_009003.1
Chromosome Location	3q13.13
Function	molecular_function;
MIM	604371

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Customer Reviews

Customer Q&As

What are the unique applications of the Human HHLA2 Stable Cell Line - HEK293T in studying human immune responses?

A: The Human HHLA2 Stable Cell Line - HEK293T, due to its stable expression of human major histocompatibility complex class II molecules (HLA-DR), holds significant value. This cell line can be used to investigate antigen presentation mechanisms, particularly how specific HLA-DR molecules bind to antigenic peptides and influence T cell recognition and activation. Additionally, it can be used for the development and screening of vaccines and immunotherapies targeting antigens restricted by specific HLA-DR alleles.

How does the Human HHLA2 Stable Cell Line - HEK293T assist researchers in predicting the immunogenicity of drugs during drug screening?

A: The Human HHLA2 Stable Cell Line - HEK293T can help researchers assess the immunogenicity of drugs by simulating the interaction between drugs and HLA-DR molecules. By monitoring peptides that bind to HLA-DR, researchers can predict whether drug molecules are likely to elicit an immune response, allowing for the early screening of potentially immunogenic drugs and avoiding immune-related side effects in subsequent clinical trials.

What are the potential applications of the Human HHLA2 Stable Cell Line - HEK293T in studying autoimmune diseases?

A: The Human HHLA2 Stable Cell Line - HEK293T can be used to investigate antigen presentation abnormalities in autoimmune diseases. By analyzing the expression and function of HLA-DR molecules in these cells, scientists can better understand how self-antigens are mistakenly recognized as foreign antigens, leading to autoimmune responses. This is significant for developing new treatment strategies, such as modulating HLA-DR molecules to reduce the pathological progression of autoimmune diseases.

How does the Human HHLA2 Stable Cell Line - HEK293T assist scientists in understanding the binding characteristics of antigenic peptides to HLA-DR molecules in vaccine development?

A: The Human HHLA2 Stable Cell Line - HEK293T provides a platform for studying how antigenic peptides bind to HLA-DR molecules and how this binding affects T cell activation. By using this cell line, scientists can screen for antigenic peptides that effectively bind to HLA-DR and activate T cells, which is crucial for designing efficient vaccines.

What are the advantages of the Human HHLA2 Stable Cell Line - HEK293T in studying the epitope specificity of HLA-DR molecules?

A: A significant advantage of the Human HHLA2 Stable Cell Line - HEK293T is its ability to stably express a specific HLA-DR molecule, allowing researchers to focus on the interactions between this particular molecule and antigenic peptides. This specificity helps reveal the epitope specificity of HLA-DR molecules, how they recognize and present specific antigen fragments, which is important for understanding the specificity of immune responses and developing personalized medical strategies.

What are the potential applications of the Human HHLA2 Stable Cell Line - HEK293T in studying drug metabolism and drug-drug interactions?

A: The Human HHLA2 Stable Cell Line - HEK293T can be used to study how drugs are metabolized within cells and how drug molecules interact with HLA-DR molecules, affecting their distribution and clearance in the body. This is crucial for predicting drug pharmacokinetic properties and potential drug-drug interactions, especially in the context of polypharmacy.

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