Cat.No. : CSC-RO0524 Host Cell: HEK293T
| Cat. No. | CSC-RO0524 |
| Description | This cell line is derived from HEK293T and is engineered to stably overexpress Human HHLA2. |
| Gene | HHLA2 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human HHLA2 Stable Cell Line - HEK293T, due to its stable expression of human major histocompatibility complex class II molecules (HLA-DR), holds significant value. This cell line can be used to investigate antigen presentation mechanisms, particularly how specific HLA-DR molecules bind to antigenic peptides and influence T cell recognition and activation. Additionally, it can be used for the development and screening of vaccines and immunotherapies targeting antigens restricted by specific HLA-DR alleles.
A: The Human HHLA2 Stable Cell Line - HEK293T can help researchers assess the immunogenicity of drugs by simulating the interaction between drugs and HLA-DR molecules. By monitoring peptides that bind to HLA-DR, researchers can predict whether drug molecules are likely to elicit an immune response, allowing for the early screening of potentially immunogenic drugs and avoiding immune-related side effects in subsequent clinical trials.
A: The Human HHLA2 Stable Cell Line - HEK293T can be used to investigate antigen presentation abnormalities in autoimmune diseases. By analyzing the expression and function of HLA-DR molecules in these cells, scientists can better understand how self-antigens are mistakenly recognized as foreign antigens, leading to autoimmune responses. This is significant for developing new treatment strategies, such as modulating HLA-DR molecules to reduce the pathological progression of autoimmune diseases.
A: The Human HHLA2 Stable Cell Line - HEK293T provides a platform for studying how antigenic peptides bind to HLA-DR molecules and how this binding affects T cell activation. By using this cell line, scientists can screen for antigenic peptides that effectively bind to HLA-DR and activate T cells, which is crucial for designing efficient vaccines.
A: A significant advantage of the Human HHLA2 Stable Cell Line - HEK293T is its ability to stably express a specific HLA-DR molecule, allowing researchers to focus on the interactions between this particular molecule and antigenic peptides. This specificity helps reveal the epitope specificity of HLA-DR molecules, how they recognize and present specific antigen fragments, which is important for understanding the specificity of immune responses and developing personalized medical strategies.
A: The Human HHLA2 Stable Cell Line - HEK293T can be used to study how drugs are metabolized within cells and how drug molecules interact with HLA-DR molecules, affecting their distribution and clearance in the body. This is crucial for predicting drug pharmacokinetic properties and potential drug-drug interactions, especially in the context of polypharmacy.
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