Cat.No. : CSC-RO0568 Host Cell: HEK293T
| Cat. No. | CSC-RO0568 |
| Description | This cell line is derived from HEK293T and is engineered to stably overexpress Human FOLR1. |
| Gene | FOLR1 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Folate deficiency is linked to impaired embryo development, leading to an elevated risk of fetal neural tube defects and spontaneous abortion, underscoring the crucial role of folates in embryonic development. Researchers observed the presence of FOLR1 protein during mouse and human ovarian follicle development, as well as in 2-cell mouse embryos. FOLR1 was highly enriched in oocytes and granulosa cells of mouse follicles, and specifically present in oocytes of human primary and intermediate follicles. The distribution of FOLR1 was noted as membrane-enriched in follicles, with cytoplasmic localization in oocytes and granulosa cells. FOLR1-eGFP fusion protein in 2-cell embryos exhibited a similar distribution pattern. Knock-down of Folr1 mRNA function using siRNA reduced the developmental capacity of embryos, indicating the importance of maternally contributed protein and zygotic transcripts in sustaining embryonic development.
Figure 1. The dynamic intracellular distribution of FOLR1 was assessed by fusing it with eGFP, allowing researchers to track its dynamics and distribution in mouse 2-cell embryos via confocal imaging. (Strandgaard T, et al., 2017)
A: The HEK293T cell line originates from human embryonic kidney cells, genetically transformed to express the SV40 large T-antigen.
A: Stability of FOLR1 expression was confirmed through periodic assessment via flow cytometry or immunofluorescence assays over multiple passages.
A: Quality control involved rigorous testing for mycoplasma contamination, confirmation of stable transgene integration, and assessment of phenotypic consistency.
A: HEK293T cells offer high transfection efficiency, robust growth characteristics, and a well-characterized genomic background, ensuring reliable and consistent expression of FOLR1.
A: Expression levels of FOLR1 in the stable cell line are typically quantified and compared to physiological levels in relevant human tissues, ensuring biological relevance and functionality.
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This cell line rocks! With its robust FOLR1 expression, my experiments are more reliable than ever. No more guesswork!
Wow, using this cell line has been a game-changer. It's like having a spotlight on FOLR1, making my research clearer and more focused.
Seriously impressed! The stable expression of FOLR1 in HEK293T cells has made studying folate receptors a breeze. No more ups and downs in expression levels!
Can't recommend this cell line enough! Thanks to its strong FOLR1 expression, I've got consistent results, making my findings more trustworthy.
Love how this cell line simplifies my work! With FOLR1 expression locked in, I can dive deeper into folate receptor biology without any worries.
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