Cat.No. : CSC-RO0359 Host Cell: Ba/F3
Size: >1x10^6 frozen cells/vial, 1 mL Stability: Stable in culture over a minimum of 10 passages
| Cat. No. | CSC-RO0359 |
| Description | This cell line is engineered to stably overexpress exogenous human ETV6-NTRK2 fusion protein. |
| Target Gene | ETV6-NTRK2 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | Ba/F3 |
| Host Cell Species | Mus musculus (Mouse) |
| Stability | Stable in culture over a minimum of 10 passages |
| Application | Drug screening and biological assays |
| Growth Conditions | 37 °C, 5% CO2 |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Size | >1x10^6 frozen cells/vial, 1 mL |
| Biosafety Level | 2 |
| Thawing & Subculturing Instructions | 1. Thaw cells by gently swirling in a 37°C water bath. To limit contamination, do not submerge the O-ring and cap. 2. When cells are ~70% thawed (~1 min), transfer the vial into a biosafety cabinet, and wipe the surface with 70% ethanol. Allow tube to dry completely. 3. Transfer the cells gently into a 15 mL conical tube containing 10 mL of pre-warmed culture medium (without antibiotic selection marker). Centrifuge cells at ~125 x g for 5~7 min. 4. Remove supernatant without disturbing the pellet, and resuspend cells in 1 mL culture medium (without antibiotic selection marker). Transfer cells to a 6-well plate containing ~2 mL pre-warmed growth medium (without antibiotic selection marker) or a T25 flask containing 5 mL pre-warmed culture medium (without antibiotic selection marker). 5. Incubate the culture at 37°C with 5% CO2. 6. Subculture: split saturated culture 1:4 ~ 1:6 every 3 days; seed out at about 1~3 x 10^5 cells/mL. |
| Growth Properties | Suspension, round |
| Freeze Medium | Frozen with 70% medium, 20% FBS, 10% DMSO |
| Freezing Instructions | Cells are recommended to generate additional frozen stocks at early passages. Frozen stocks should be preserved in a designated cryopreservation medium or in 70% RPMI 1640 + 20% FBS + 10% DMSO (without antibiotic selection marker). 1. Prepare the freezing medium (70% RPMI 1640 + 20% FBS + 10% DMSO, without antibiotic selection marker) fresh immediately before use. 2. Keep the freezing medium on ice and label cryovials. 3. Transfer cells to a sterile, conical centrifuge tube, and count the cells. 4. Centrifuge the cells at 250 x g for 5 minutes at room temperature and carefully aspirate off the medium. 5. Resuspend the cells at a density of at least 3 x10^6 cells/ml in chilled freezing medium. 6. Aliquot 1 ml of the cell suspension into each cryovial. 7. Freeze cells in the CoolCell freezing container overnight in a -80°C freezer. 8. Transfer vials to liquid nitrogen for long-term storage. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Next-generation sequencing (NGS) for the detection of molecular changes has advanced as a result of targeted therapy in conjunction with companion diagnostics. To detect NTRK1, NTRK2, NTRK3, ROS1, and ALK rearrangements in formalin-fixed paraffin-embedded clinical tissues, the researchers have created a two-step diagnostic technique. To identify samples expressing TrkA, TrkB, TrkC, ROS1, and ALK, this test employs pan-receptor tyrosine kinase cocktail immunohistochemistry screening. An RNA-based anchored multiplex polymerase chain reaction NGS assay is performed on positive samples. Gene rearrangements can be reliably and accurately identified using the NGS assay. The confidence in negative NGS results is ensured by implementing an RNA quality control metric. With a 100% negative predictive value, the pan-receptor tyrosine kinase labeling raises the detection rate of gene rearrangements from 4% to 9%.
Figure 1. To investigate gene rearrangements, the scientists prepared FFPE specimens from cell pellets embedded in agarose at densities of 100–200 million cells, using the ETV6-NTRK2 stable cell line (BaF3). The researchers utilized the ETV6-NTRK2 Stable Cell Line - BaF3 to investigate gene rearrangements using NGS assays, examining median unique RNA reads for receptors like NTRK1, NTRK2, NTRK3, ROS1, and ALK. (Murphy DA, et al., 2017)
A: Our human ETV6-NTRK2 stable cell line has undergone rigorous purification and validation procedures to ensure its purity and stability. We use a variety of techniques to identify cell lines, including PCR, immunofluorescence staining, and Western blot. We also regularly perform cytological testing of cell lines to ensure they are free of hybridization or mutation.
A: Our human ETV6-NTRK2 stable cell line has stable expression levels and has been widely used in research on the mechanisms of leukemia. We provide detailed experimental data support, including Western blot analysis, flow cytometry, etc. Customers can refer to these data to evaluate the performance of our products.
A: Our human ETV6-NTRK2 stable cell line has been used in new drug development experiments. We provide relevant literature support demonstrating the application potential of our products in these areas. Customers can refer to these documents to understand the performance and application range of our products.
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I would like to commend the supplier for the professional service they provided. Their expertise on the product was impressive and they gave me detailed answers to my questions, which gave me a clearer understanding of the product.
I am very pleased with the stability of the cell line. During the culture process, I observed healthy cell growth and stable gene expression, which provided reliable data support for my experimental results. Overall, I am very satisfied with the quality and service of this product and look forward to more cooperation opportunities in the future.
My experience with the Human ETV6-NTRK2 stable cell line has been excellent. They were knowledgeable about the product and were able to provide timely and effective support, making my research progress smoothly. I am also very pleased with the stability of the cell line. During the culture process, I observed stable cell growth and consistent gene expression, which provided reliable experimental data for my research.
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