Human DNMT3B Knockout Cell Line-HEK293T

Name: Human DNMT3B Knockout Cell Line-HEK293T
SKU: CSC-RT2710
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT2710

Target Gene : DNMT3B Host Cell : HEK293T

Size : >1x10⁶ cells/vial Validation : Sequencing

Inquire for Price

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT2710
Description	This cell is a stable cell line with a homozygous knockout of human DNMT3B using CRISPR/Cas9.
Target Gene	DNMT3B
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Size	1 vial (>10^6 cell/vial)
Validation	Sequencing
Storage	Liquid nirtogen
Shipping	Dry ice package

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	DNMT3B DNA (cytosine-5-)-methyltransferase 3 beta [ Homo sapiens ]
Gene Symbol	DNMT3B
Synonyms	ICF; ICF1; M.HsaIIIB
Gene Description	DNA (cytosine-5-)-methyltransferase 3 beta
GeneID	1789
Uni ProtID	Q9UBC3
mRNA Refseq	NM_175848.1
Protein Refseq	NP_787044.1
Chromosome Location	20q11.2
Function	DNA (cytosine-5-)-methyltransferase activity; DNA (cytosine-5-)-methyltransferase activity; DNA (cytosine-5-)-methyltransferase activity; DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates; DNA-methyltransferase activity; metal ion binding; protein binding; transcription corepressor activity; transcription corepressor activity; unmethylated CpG binding;
Pathway	Cysteine and methionine metabolism, organism-specific biosystem; Cysteine and methionine metabolism, conserved biosystem; Methionine degradation, organism-specific biosystem; Methionine degradation, conserved biosystem; One Carbon Metabolism, organism-specific biosystem;
MIM	602900

Quick Inquiry

Background

Applications

Q & A

Customer Reviews

DNMT3B (DNA (cytosine-5)-methyltransferase 3 beta) encodes an enzyme that plays an important role in epigenetic regulation of the human genome. The enzyme is particularly important in the process of de novo methylation, which involves the addition of methyl groups to DNA cytosine residues. DNA methylation is a key epigenetic modification that contributes to a variety of cellular processes, including embryonic development, genomic imprinting, and X-chromosome inactivation. Modifications performed by DNMT3B can affect gene expression patterns by altering chromatin structure, making genes easier or harder to transcribe.

Mutations in the DNMT3B gene are associated with a rare genetic disorder called immunodeficiency-centromere instability-facial anomalies (ICF) syndrome. ICF syndrome is characterized by defects in lymphocyte maturation, centromere instability, and distinct facial anomalies. Recent studies have also implicated DNMT3B in various forms of cancer. Abnormal methylation patterns due to dysregulated DNMT3B function can lead to silencing of tumor suppressor genes and activation of oncogenes, which can lead to tumor initiation and progression. Studies have shown that overexpression of DNMT3B is frequently observed in several types of cancer, including lung, colon, and breast cancer.

Human DNMT3B knockout cell lines derived from HEK293T cells are powerful tools for epigenetic research, especially in studies investigating the role of DNA methylation. DNMT3B is one of the major DNA methyltransferases involved in establishing genomic DNA methylation patterns during development. Here are some of the key applications of DNMT3B knockout cell lines:

Cancer Research: Knockout cell lines help researchers understand the role of DNMT3B in tumorigenesis and cancer progression by revealing the consequences of its absence, thereby aiding in the development of targeted cancer therapies.

Genetic and Epigenetic Research: HEK293T cells with DNMT3B knockout facilitate the study of epigenetic modifications, such as DNA methylation, which play a crucial role in gene regulation, development, and disease. This provides further insight into gene expression patterns and the mechanisms underlying various genetic diseases.

Drug Screening and Development: These knockout cell lines provide a powerful platform for high-throughput drug screening. By evaluating the response of cells to different compounds in the absence of DNMT3B, researchers can identify potential drug candidates and evaluate their mechanisms of action, thereby aiding in the development of new therapeutic approaches.

Stem Cell Research: DNMT3B is essential for maintaining the pluripotency and differentiation potential of stem cells. Knockout lines are important models for studying how loss of DNMT3B affects stem cell maintenance and differentiation, providing crucial insights into regenerative medicine and developmental biology.

Customer Q&As

What is the recommended growth medium? Does it require antibiotic selection?

A: DMEM supplemented with 10% fetal bovine serum. <br> It is not required to add the selection antibiotics when culturing the KO cells.

How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Question *

Name

Country *

Email *

Customer Reviews

Excellent Customer Support

Creative Biogene team provided comprehensive documentation and was readily available to answer any technical queries we had. It made the transition to using this cell line seamless and trouble-free.

United Kingdom

2020-01-16

Versatile application

We have utilized the DNMT3B knockout HEK293T cells in a variety of applications ranging from gene expression studies to drug screening assays. It's a highly recommended tool for any lab focused on epigenetics or gene regulation.

Canada

2023-04-26

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Product Name *

Catalog Name *

Rating

Needs improvement

Satisfaction

General satisfaction

Very satisfaction

Product Review Title *

Summary *

Name

Country *

Email *

Related Products

Cat.No.	Product Name	Price
CSC-RK0053	Nos3 Knockout Mouse Lung Fibroblasts	Inquiry
CSC-RK0132	Ncf1 Knockout Mouse Liver Cells	Inquiry
CSC-RK0062	Nos3 Knockout Mouse Liver Cells	Inquiry
CSC-RK0100	Icam1 Knockout Mouse Spleen Cells	Inquiry
CSC-RK0111	Cybb Knockout Mouse Bone Marrow Mesenchymal Stem Cells	Inquiry
CSC-RK0081	Tlr4 Knockout Mouse Kidney Cells	Inquiry
CSC-RK0077	Tlr4 Knockout Mouse Embryonic Stem Cells	Inquiry
CSC-RK0058	Nos3 Knockout Mouse Embryonic Stem Cells	Inquiry
CSC-RK0110	Cybb Knockout Mouse Bone Marrow Neutrophils	Inquiry
CSC-RK0064	Nos3 Knockout Mouse Lung Cells	Inquiry

Human DNMT3B Knockout Cell Line-HEK293T

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Background

Applications

Q & A

Customer Reviews

What is the recommended growth medium? Does it require antibiotic selection?

How is the knockout cell line validated?

Is the product a single clonal cell or mixed cell pool?

Can I confirm gene knockout by RT-qPCR?

How can I store the cell product?

Is it possible to get multiple knockout clones for my GOI?

Excellent Customer Support

Versatile application

Related Products

Related Services