Cat.No. : CSC-RO0660 Host Cell: HEK293T
| Cat. No. | CSC-RO0660 |
| Description | This cell line is engineered to stably co-express human angiotensin I converting enzyme 2 (ACE2) and human transmembrane serine protease 2 (TMPRSS2) in HEK293T cells. |
| Gene | ACE2/TMPRSS2 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Study the mechanisms of viral entry and replication 2. Research and in vitro screening of potential drugs 3. Vaccine development 4. The production of pseudotyped viruses |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A crucial component was found to be LRRC15, a TLR-related cell surface receptor, by whole-genome CRISPR activation screening. LRRC15 expression is elevated in lung myofibroblasts during severe COVID-19 infection and increases SARS-CoV-2 spike binding. While LRRC15 does not directly promote viral infection, it can inhibit the expression of antiviral factors, decrease collagen synthesis, and dampen SARS-CoV-2 infection in fibroblasts. According to this study, LRRC15 is a new spike-binding receptor for SARS-CoV-2 that is essential for regulating antiviral and antifibrotic responses as well as viral load management.
Figure 1. The researchers generated HEK293T-ACE2 stable cell lines by transducing HEK293T cells with an ACE2-expressing lentivirus, followed by immunostaining and single-cell sorting. They then transduced HEK293T-ACE2 cells with TMPRSS2-expressing lentivirus to create HEK293T-ACE2-TMPRSS2 clones, selecting a highly permissive clone for further experiments. Then, the researchers utilized HEK293T-ACE2-TMPRSS2 cells to confirm LRRC15 binding to SARS-CoV-2 spike protein via flow cytometry and immunoprecipitation, demonstrating interaction between LRRC15-GFP and spike protein, and showing LRRC15's role in viral binding and entry. (Loo L, et al., 2023)
A: The cell line co-expresses both ACE2 and TMPRSS2, which are essential for SARS-CoV-2 entry into host cells. By studying the interactions between these two proteins, researchers can gain insights into the coordinated host cell response to viral infection and identify potential targets for therapeutic intervention.
A: The cell line can be used to test the ability of candidate vaccines and adjuvants to prevent SARS-CoV-2 entry into host cells. By evaluating the immune response elicited by these candidates, researchers can optimize vaccine formulations to enhance protection against the virus.
A: The cell line can be employed in vaccine development by testing the ability of candidate vaccines to prevent viral entry and replication. By evaluating the immune response elicited by these vaccines, researchers can optimize their formulation and delivery methods to enhance protection against SARS-CoV-2.
A: This cell line can be used in high-throughput screening assays to identify compounds that effectively inhibit SARS-CoV-2 entry or block the interaction between the virus and its human receptors. It allows for the rapid evaluation of drug candidates and their potential side effects on human cells.
A: The cell line can be adapted to study interactions with other coronaviruses or pathogens that use similar host receptors for entry. This can provide insights into the broader mechanisms of viral pathogenesis and inform the development of broad-spectrum antiviral strategies.
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The Human ACE2/TMPRSS2 Stable Cell Line - HEK293T features high transfection efficiency, meaning it can effectively take up and express foreign genetic material. This characteristic is crucial for research requiring gene overexpression or knockout, as it ensures consistent and reliable experimental results across various applications, particularly in studying viral entry mechanisms.
This cell line consistently expresses the ACE2 receptor and TMPRSS2 protease, pivotal for SARS-CoV-2 studies. The robust expression levels in Human ACE2/TMPRSS2 Stable Cell Line - HEK293T facilitate detailed investigations into the virus-host interactions and can accelerate the development of therapeutic strategies targeting these proteins.
Human ACE2/TMPRSS2 Stable Cell Line - HEK293T is versatile, suitable for a wide range of research applications, including virology, toxicology, and pharmacology. This versatility stems from the cell line's robust handling and maintenance characteristics, allowing us to use it in diverse experimental setups.
Accompanying the Human ACE2/TMPRSS2 Stable Cell Line - HEK293T is comprehensive documentation and technical support. This support ensures that we can maximize the utility of the cell line, addressing both routine and complex experimental scenarios with expert guidance.
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