Cat.No. : CSC-SC000069-1 Host Cell: MDCK-II
| Cat. No. | CSC-SC000069-1 |
| Description | This cell line is engineered to stably overexpress human ATP binding cassette subfamily G member 2 (Junior blood group) (ABCG2). |
| Gene | ABCG2 |
| Host Cell | MDCK-II |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
In bovine mammary glands, the ABCG2 transporter plays an active role in pumping xenobiotics into dairy milk. The researchers investigated the effects of 13 commonly used pesticides in Europe on AhR activation and bABCG2 efflux activity using MDCKII cells expressing mammary bABCG2. Pesticides were applied at concentrations equivalent to maximum residue levels (MRLs) in bovine fat or muscle. Prochloraz, a known AhR activator, served as a positive control, and tolclofos-methyl as a negative control. Chlorpyrifos-methyl, diflufenican, ioxynil, rimsulfuron, and tebuconazole significantly increased CYP1A1 mRNA levels, CYP1A activity, and bABCG2 efflux compared to controls. Conversely, dimethoate, dimethomorph, glyphosate, iprodione, methiocarb, and thiacloprid showed no AhR activation or bABCG2 induction. MDCKII-bABCG2 cells proved effective in identifying AhR- and bABCG2-inducing pesticides, offering an in vitro alternative to animal-based studies for pesticide risk assessment.
Figure 1. The researchers assessed MDCKII-bABCG2 cells' response to AhR-activating pesticides. After 24-hour exposure at 10-fold MRL concentrations, Hoechst 33342 accumulation was measured with or without the ABCG2-inhibitor Ko143 (5 μM). (Kuhnert L, et al., 2020)
A: The Human ABCG2 Stable Cell Line - MDCKII was established by transfecting the human ABCG2 gene into MDCKII (Madin-Darby Canine Kidney II) cells. MDCKII cells are a canine kidney cell line commonly used in vitro studies. The human ABCG2 gene encodes a multidrug resistance protein, and its transfection into MDCKII cells allows for the expression of the ABCG2 protein, thus creating this stable cell line.
A: The expression level of the ABCG2 gene in the Human ABCG2 Stable Cell Line - MDCKII is significantly higher than in the original MDCKII cells. By transfecting the human ABCG2 gene, the cells acquire additional copies of the ABCG2 gene, resulting in increased expression levels of this gene and consequently higher production of the multidrug resistance protein.
A: The Human ABCG2 Stable Cell Line - MDCKII exhibits pronounced multidrug resistance characteristics, primarily due to its high-level expression of the ABCG2 protein. ABCG2 is an ATP-binding cassette transporter protein that forms channels on the cell membrane, facilitating the efflux of various drugs from the cell and conferring multidrug resistance.
A: The Human ABCG2 Stable Cell Line - MDCKII can be employed in high-throughput screening of potential drug resistance reversal agents. By exposing these cells to candidate compounds and observing changes in their sensitivity to drugs, promising compounds capable of reversing multidrug resistance can be identified.
A: The ABCG2 protein in the Human ABCG2 Stable Cell Line - MDCKII possesses a transmembrane structure, forming channels on the cell membrane. Its structural features include two nucleotide-binding domains and multiple transmembrane regions, collectively constituting the functional domain of the ABCG2 protein.
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The Human ABCG2 Stable Cell Line - MDCKII is an ideal model for studying the drug efflux mechanism mediated by ABCG2.
This cell line can be used to evaluate the sensitivity of drugs to ABCG2-mediated multidrug resistance.
Contributes to the analysis of drug interactions with the ABCG2 protein and their impact on drug efficacy.
Utilizes the MDCKII cell line to study the function of ABCG2 in biomembrane formation and maintenance.
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