HBV-B/adw AAV (Serotype 8)

Name: HBV-B/adw AAV (Serotype 8)
SKU: AAVH004Z

Cat.No. : AAVH004Z

Titer: ≥1x10^12 GC/mL / ≥1x10^13 GC/mL Size: 30 ul/100 ul/500 ul/1 ml

Serotype: AAV Serotype 8 Storage: -80 ℃

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AAV Particle Information

Quality Control

Cat. No.	AAVH004Z
Description	AAV serotype 8 (AAV8) viral particles containing genome DNA of Hepatitis B virus (HBV) genotype B, serotype adw.
Serotype	AAV Serotype 8
Product Type	AAV viral particles
Application	AAV serotype 8 (AAV8) viral particles contain genomic DNA from the hepatitis B virus (HBV) genotype B, serotype adw. Here are some potential applications: Gene therapy for liver disease: Given the strong tropism of AAV serotype 8 for hepatocytes, it is often used to deliver therapeutic genes to treat liver diseases, including inherited diseases such as hemophilia, hyperbilirubinemia, and other metabolic liver diseases. Hepatitis B research: Since HBV is involved, one application could be to create models to study hepatitis B and its interactions with hepatocytes. This could help researchers better understand the disease and develop potential treatments or vaccines. Vaccine development: The combination could be used to develop a vaccine against hepatitis B by delivering specific antigens to stimulate an immune response. Gene editing: AAV vectors are also used to deliver CRISPR/Cas9 components for gene editing. This could be applied to correct genetic mutations directly within hepatocytes. Cancer treatment: Some AAV vectors are being studied for delivering genes to treat liver cancer or inhibit tumor growth.
Applications	Cell-based HBV assays, developing HBV infection mouse models
Insert	Genome DNA of Hepatitis B virus (HBV) genotype B, serotype adw
Titer	Varies lot by lot, typically ≥1x10^12 GC/mL
Size	Varies lot by lot, for example, 30 μL, 50 μL, 100 μL etc.
Storage	Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping	Frozen on dry ice

Creative Biogene ensures high-quality AAV particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between AAV particle lots.
Endotoxin	Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in the production of AAV, especially for applications in animal studies and gene therapy. Effective endotoxin quality control is essential in the development and manufacturing of AAV particles. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced AAV particles to ensure regulatory compliance.
Purity	AAV purity is critical for ensuring the safety and efficacy of AAV-based applications.AAV capsids are composed of three main protein components, known as viral proteins: VP1, VP2, and VP3. These proteins play a critical role in the structure and functionality of the AAV capsid. Monitoring the VP1, VP2, and VP3 content in AAV preparations is essential for quality control in AAV production. Our AAV particles are tested for showing three clear bands of VP1, VP2 VP3 by SDS-PAGE.
Sterility	The AAV virus samples are inoculated into the cell culture medium for about 5 days to detect bacterial and fungal growth.
Transducibility	Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of AAV to deliver genetic material into target cells or tissues, and assess gene expression and functional activities.
Empty vs. Full Capsids	Based-on our proprietary AAV production and purification technology, Creative Biogene can always offer AAV particles with high ratio of full capsids. If required, we can also assess the ratio for a specifc lot of AAV particles by transmission electron microscopy (TEM) or other methods.

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Hepatitis B virus (HBV) is a member of the Hepadnaviridae family of viruses. The genome of HBV is made of a partially double-stranded DNA virus. There are four known genes encoded by the genome called C, P, S, and X. HBV is divided into four major serotypes (adr, adw, ayr, ayw) based on antigenic epitopes present on its envelope proteins, and is divided into ten genotypes (A to J) and forty subgenotypes according to overall nucleotide sequence variation of the genome. HBV infection can cause the disease hepatitis B. Hepatitis B can be acute and later become chronic, leading to other diseases and health conditions. Infection with HBV can also lead to cirrhosis and hepatocellular carcinoma, and increase the risk of pancreatic cancer.

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