hAAT-HDV-I AAV (Serotype 8)

Name: hAAT-HDV-I AAV (Serotype 8)
SKU: AAVH005Z
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : AAVH005Z

Serotype : AAV Serotype 8 Storage : -80 ℃

Titer: Size:

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Virus Particles Information

Quality Control

Cat. No.	AAVH005Z
Description	AAV serotype 8 (AAV8) viral particles containing 1.2 copies of the full-length genome DNA of hepatitis delta virus genotype I (HDV-I) and hAAT liver-specific promoter. This product can be used in combination with the AAV-HBV series to establish HBV/HDV co-infection.
Product Type	AAV viral particles
Serotype	AAV Serotype 8
Insert	Genome DNA of hepatitis delta virus genotype I (HDV-I) and hAAT liver-specific promoter
Applications	Cell-based HDV assays, developing HDV infection mouse models
Titer	Varies lot by lot, typically ≥1x10^12 GC/mL
Size	Varies lot by lot, for example, 30 μL, 100 μL, 500 μL etc.
Storage	Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping	Frozen on dry ice

Summary	Creative Biogene ensures high-quality AAV particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between AAV particle lots.
Endotoxin	Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in the production of AAV, especially for applications in animal studies and gene therapy. Effective endotoxin quality control is essential in the development and manufacturing of AAV particles. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced AAV particles to ensure regulatory compliance.
Purity	AAV purity is critical for ensuring the safety and efficacy of AAV-based applications.AAV capsids are composed of three main protein components, known as viral proteins: VP1, VP2, and VP3. These proteins play a critical role in the structure and functionality of the AAV capsid. Monitoring the VP1, VP2, and VP3 content in AAV preparations is essential for quality control in AAV production. Our AAV particles are tested for showing three clear bands of VP1, VP2 VP3 by SDS-PAGE.
Sterility	The AAV virus samples are inoculated into the cell culture medium for about 5 days to detect bacterial and fungal growth.
Transducibility	Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of AAV to deliver genetic material into target cells or tissues, and assess gene expression and functional activities.
Empty vs. Full Capsids	Based-on our proprietary AAV production and purification technology, Creative Biogene can always offer AAV particles with high ratio of full capsids. If required, we can also assess the ratio for a specifc lot of AAV particles by transmission electron microscopy (TEM) or other methods.

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Hepatitis D virus (HDV) is an enveloped small single-stranded negative sense RNA virus with a 35–37 nm diameter and belongs to the genus, Deltaviridae from the family Kolmioviridae. HDV requires the presence of hepatitis B virus (HBV) for its assembly and replication. The virion of HDV is composed of an outer lipoprotein envelope made of the surface antigen of the HBV (HBsAg) and an inner ribonucleoprotein structure in which the HDV genome resides. HDV produces one protein with two forms, a 27 kDa large-HDAg (delta-Ag-L), and a small-HDAg of 24 kDa (delta-Ag-S). HDV is known as a "satellite virus" because it can only infect people who are also infected by the hepatitis B virus (HBV). HDV can cause severe symptoms and serious illness that can lead to liver damage and even death.

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