Cat.No. : CSC-RR0459 Host Cell: MM1.S
| Cat. No. | CSC-RR0459 |
| Description | MM1.S-GFP cell line is engineered to stably overexpress GFP under a constitutive promoter. It is an ideal tool for fluorescent tracing MM1.S cells |
| Host Cell | MM1.S |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Reporter Type | Fluorescent protein |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Current therapies often overlook the influence of osteoclast-mediated cytoprotection, leading to a need for innovative approaches in drug screening. In this work, the researchers assessed the effectiveness of several medication candidates including GDC-0941 using the GFP-MM1.S cell line, which is well-known for its sensitivity to dexamethasone. Overcoming the protective effects of osteoclasts and bone marrow stromal cells, this pan-PI3K inhibitor was shown to increase the efficacy of current therapies, hence enhancing therapeutic results for patients with active bone disease.
Figure 1. The researchers utilized eGFP-MM1.S cells to evaluate the effects of GDC-0941 on overcoming mesenchymal stromal cell-mediated cytoprotection against dexamethasone in multiple myeloma. (Kikuchi H, et al., 2023)
Creative Biogene's GFP Stable Cell Line-MM1.S is designed for researchers exploring the complex interactions between myeloma cells and their microenvironment. Our cell line allows for high-throughput screening of drug candidates and is ideal for studies focusing on osteoclast interactions and resistance mechanisms.
A: The GFP Stable Cell Line-MM1.S is characterized by its ability to constitutively express green fluorescent protein (GFP) under the control of a stable promoter. This feature allows researchers to visualize cellular processes in real-time without the need for additional labeling, making it an invaluable tool for live-cell imaging. Additionally, the cell line's stability ensures consistent GFP expression over multiple passages, which is crucial for longitudinal studies.
A: By fusing the gene of interest to the GFP gene in the GFP Stable Cell Line-MM1.S, researchers can create a cell line that expresses a fluorescently tagged protein. This allows for the direct observation of the protein's subcellular localization, trafficking, and interactions with other cellular components. Furthermore, through the use of fluorescence resonance energy transfer (FRET) or fluorescence recovery after photobleaching (FRAP) techniques, the dynamics of protein-protein interactions can be studied in living cells.
A: To maintain robust GFP expression in the GFP Stable Cell Line-MM1.S, it is essential to culture the cells in a controlled environment that mimics physiological conditions. This includes maintaining an optimal temperature of 37°C, a humidified atmosphere with 5% CO2, and using a suitable growth medium that contains all necessary nutrients and growth factors. Regular passaging and monitoring of cell density are also crucial to prevent overgrowth, which can lead to decreased GFP expression levels.
A: The GFP Stable Cell Line-MM1.S can be exposed to different stressors or therapeutic agents to study their effects on cellular behavior. The GFP tag allows researchers to monitor changes in cell morphology, motility, and apoptosis in response to these treatments. Additionally, by combining GFP expression with other reporters or markers, researchers can assess the activation of specific signaling pathways or the induction of stress responses, providing valuable insights into the mechanisms of cellular adaptation and resistance.
A: The GFP Stable Cell Line-MM1.S can be employed in high-throughput screening assays to identify compounds that modulate cellular processes related to disease progression. The GFP tag facilitates the rapid assessment of compound effects on cell viability, proliferation, and differentiation. Moreover, by integrating the GFP expression with other reporter systems, researchers can develop cell-based assays to evaluate the efficacy and toxicity of potential therapeutic agents, thereby accelerating the drug discovery process.
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The stable GFP expression simplifies cell sorting and selection via fluorescence-activated cell sorting (FACS), facilitating the enrichment or depletion of MM1.S cells in experimental setups.
GFP in the GFP Stable Cell Line - MM1.S allows for live-cell tracking, providing insights into cell behavior, migration, and interaction without the need for additional stains or dyes.
The cell line offers stable, continuous GFP expression, ensuring uniform fluorescence for consistent tracking and analysis across multiple experiments and passage numbers.
Designed for multiple myeloma studies, the GFP Stable Cell Line - MM1.S provides a specialized model to investigate this particular cancer, aiding in understanding disease mechanisms and testing therapeutics.
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