Cat.No. : CSC-RR0462 Host Cell: Colon26
| Cat. No. | CSC-RR0462 |
| Description | Colon26-GFP/Luc is derived from Colon26 cell line which has been transduced by two lentiviruses, encoding firefly luciferase and EGFP respectively, followed by antibiotic selection of stable cells. This cell line is an ideal positive control for use in both fluorescence and bioluminescence assays. |
| Host Cell | Colon26 |
| Host Cell Species | Mus musculus (Mouse) |
| Stability | Validated for at least 10 passages |
| Reporter Type | Fluorescent protein |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Colorectal cancer (CRC) ranks third among the most commonly diagnosed cancers in men and second in women globally. Researchers have explored the potential of amine-functionalized graphene oxide nanoparticles (GO-NH2) for colorectal cancer therapy. Evaluating their impact on Colon 26 cells, we assessed ROS generation, cellular proliferation, and survival. Our findings indicate that exposure to aminated GO enhances cytotoxicity through ROS induction, DNA damage, and apoptosis, suggesting its potential as an effective anticancer agent. This research sheds light on the mechanism of action of GO-based therapies in cancer treatment.
Figure 1. Researchers evaluated the viability of Colon 26 cells after incubation with pristine and aminated GO for 24 hours. They quantified viable cells and observed significant differences compared to untreated cells. (Krasteva N, et al., 2019)
A: The stability and expression level of GFP and luciferase in the Colon 26 reporter cell line were ensured through stable transfection techniques and clonal selection. Regular monitoring using assays like fluorescence microscopy and luciferase activity assays maintained consistent expression levels.
A: The GFP/Luc Reporter Cell Line - Colon 26 allows for the investigation of various cellular processes, including tumor growth, metastasis, and response to therapy. The dual-reporter system enables real-time visualization and quantification of these processes in colorectal cancer cells.
A: The GFP/Luc Reporter Cell Line - Colon 26 serves as a valuable tool for drug screening assays in colorectal cancer research. Researchers can use this cell line to evaluate the efficacy and toxicity of potential therapeutics, accelerating the discovery and development of novel anticancer agents.
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The GFP/Luc Reporter Cell Line in Colon 26 cells offers a powerful combination of GFP fluorescence and luciferase bioluminescence, enabling comprehensive analysis of colorectal cancer biology.
The GFP/Luc Reporter Cell Line simplifies experimental workflows, allowing for efficient data collection and analysis in colorectal cancer research. Its dual-reporter system provides multifaceted data, empowering deeper understanding of tumor microenvironment dynamics and therapeutic targets.
With both reporters, this cell line facilitates detailed investigation of tumor growth, metastasis, and response to therapy, advancing our understanding of colorectal cancer progression. From studying tumor-stroma interactions to evaluating treatment efficacy, this cell line serves as an invaluable resource for unraveling the complexities of colorectal cancer biology and developing novel therapeutic strategies.
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