Cell Line Engineering

Cell line engineering is a promising optimization strategy that can help create transgenic host cell lines to produce recombinant proteins with desired properties. Creative Biogene has developed a variety of advanced cell line engineering strategies that can help customers edit any cell line, including complex cell lines, to meet different project development demands. Regardless of the type of protein to be expressed or the type of cell expressing the protein, we can provide customized strategies.

Benefits of Cell Line Engineering in Production

Optimizing the performance of cell lines to improve stability, scalability and productivity.
Making possible the production of complex molecules that are difficult or impossible to produce with conventional methods.
Improving production efficiency and reduces production costs.

Cell Line Engineering Strategies Offered by Creative Biogene

Cell engineering is mainly related to the modification and alteration of host cells to improve the yield and quality of expressed proteins. Creative Biogene offers several approaches to alter cellular processes. Examples include silencing or overexpressing target genes and altering gene expression using microRNAs (miRNAs). Once miRNA sequences are identified through the complete genome sequence, they can be overexpressed or silenced to achieve the desired regulation of gene expression. In addition, gene editing technologies for cell lineage engineering, such as CRISPR/Cas9, TALENs and zinc finger nucleases, can be used to target a variety of features, such as apoptosis, glycosylation and enzymes (e.g., dihydrofolate-inducible enzymes). Creative Biogene can leverage these advanced technologies to create specific genetic modifications in host cells through the following different strategies:

Targeted gene insertion and knockout

Our expert and skilled scientists use gene editing techniques to insert or knock out specific genes in host cell lines to improve the yield, stability or quality of recombinant proteins.

Site-directed mutagenesis

Targeted mutagenesis establishes targeted amino acid substitutions or deletions in protein sequences that affect protein function, stability, or solubility.

Gene activation or inhibition

Modulate the expression of specific genes in the host cell line by gene activation or repression techniques (e.g. RNAi or CRISPR) to increase the yield of recombinant proteins or to reduce unwanted side effects.

What We Deliver

Engineered cell lines
Quality control and test reports
Comprehensive documentation of the project process
Suggestions for further optimization or validation of engineered cell lines

Whether you are at the intersection of scale or complexity, Creative Biogene can provide expert cell line engineering services to help you move your research forward quickly. We can achieve on-time delivery, consistent quality and complete confidentiality. Please feel free to contact us if you have a need, and our experienced specialists will be happy to assist you.

* For research use only. Not intended for any clinical use.

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