Cat.No. : LV00991Z
Titer: ≥1*10^7 TU/mL / ≥1*10^8 TU/mL / ≥1*10^9 TU/mL Size: 100 ul/500 ul/1 mL
Storage: -80℃ Shipping: Frozen on dry ice
| Cat. No. | LV00991Z |
| Description | This lentivirus contains Ca2+ indicator GCaMP6f under the control of CAG promoter. |
| Target Gene | GCaMP6f |
| Titer | Varies lot by lot, for example, ≥1*10^7 TU/mL, ≥1*10^8 TU/mL, ≥1*10^9 TU/mL etc. |
| Size | Varies lot by lot, for example, 100 ul, 500 ul, 1 mL etc. |
| Storage | Store at -80℃. Avoid multiple freeze/thaw cycles. |
| Shipping | Frozen on dry ice |
| Creative Biogene ensures high-quality lentivirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between lentivirus particle lots. | |
| Mycoplasma | Creative Biogene routinely tests for mycoplasma contamination using a mycoplasma detection kit. Cell lines are maintained for approximately 20 passages before being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks after thawing, cell culture supernatants are tested for mycoplasma contamination. Creative Biogene ensures that lentiviral products are free of mycoplasma contamination. |
| Purity | Creative Biogene evaluates the level of impurities, such as residual host cell DNA or proteins, in prepared lentiviral vectors to ensure they meet quality standards. |
| Sterility | The lentiviral samples were inoculated into cell culture medium for about 5 days and the growth of bacteria and fungi was tested. Creative Biogene ensures that the lentiviral products are free of microbial contamination. |
| Transducibility | Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of lentivirus to deliver genetic material into target cells, and assess gene expression and functional activities. |
| Proviral Identity Confirmation | All Creative Biogene lentiviral vectors are confirmed to have correctly integrated provirus using PCR. This test involves transducing cells with serial dilutions of the lentiviral vector, harvesting the cells a few days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion of the expected lentiviral insert. |
In glioblastoma (GBM), the impact of an altered glycocalyx remains largely unexplored. Sialic acid, the terminal moiety of cell-coating glycans, is essential for cell-cell contacts. However, sialic acid turnover in glioma and its impact on tumor networks remain unknown. Here, visualization and quantitative analysis of newly synthesized sialic acid revealed a high incidence of de novo sialylation in GBM cells. Sialyltransferases and sialidases are highly expressed in GBM, suggesting that extensive turnover of sialic acid is associated with GBM pathology. Inhibition of sialic acid biosynthesis or desialylation affects tumor growth patterns and leads to altered network connectivity in glioblastoma cells.
Here, the researchers quantified gap junction-mediated cytosolic traffic within tumor networks by real-time imaging of fluorescence-guided cell-to-cell transfer of calcein—a fluorescent molecule that is only able to spread from cell-to-cell through intercellular gap junctions, workflow is depicted in Figure 1A. For MFA treatment, researchers observed a significant decrease in calcein receptor cells up to 150 min later (Figures 1B, C). Based on these findings of MFA-mediated functional inhibition of intercellular cytoplasmic exchange through gap junctions, calcium imaging was performed in glioblastoma cells transduced with the LV-CAG-GCaMP6f virus (Figure 1D). The researchers used 100 µM glutamate stimulation for 10 minutes to analyze network communication in tumor cells. After 5 min of recording, MFA was added, and the results showed a reduction in calcium signaling after MFA treatment (Figure 1E). Glioblastoma networks exhibited properties similar to neuronal networks, with bursts of synchronized activity, with all cells participating in network oscillations (Figure 1E).
Figure 1. Meclofenamate causes functional decoupling of glioblastoma cells. (Schneider M, et al., 2021)
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GCaMP6f’s sensitivity is outstanding! Compared to other calcium indicators, this product provided clearer activity traces with minimal background noise.
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