CAG-FLEX-jGCaMP7f AAV (Serotype 9)

Name: CAG-FLEX-jGCaMP7f AAV (Serotype 9)
SKU: AAB0044
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : AAB0044

Serotype : AAV Serotype 9 Storage : -80 ℃

Titer: Size:

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Virus Particles Information

Quality Control

Cat. No.	AAB0044
Description	Premade AAV particles in serotype 9 containing Cre-dependent jCaMP7f under the control of a CAG promoter.
Product Type	Adeno-associated virus particles
Tag	jGCaMP7f
Serotype	AAV Serotype 9
Biosensor	jGCaMP7f-Improved SNR, fast kinetics
Titer	Varies lot by lot, typically ≥1x10^12 GC/mL
Size	Varies lot by lot, for example, 30 μL, 100 μL, 500 μL etc.
Storage	Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping	Frozen on dry ice

Summary	Creative Biogene ensures high-quality AAV particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between AAV particle lots.
Endotoxin	Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in the production of AAV, especially for applications in animal studies and gene therapy. Effective endotoxin quality control is essential in the development and manufacturing of AAV particles. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced AAV particles to ensure regulatory compliance.
Purity	AAV purity is critical for ensuring the safety and efficacy of AAV-based applications.AAV capsids are composed of three main protein components, known as viral proteins: VP1, VP2, and VP3. These proteins play a critical role in the structure and functionality of the AAV capsid. Monitoring the VP1, VP2, and VP3 content in AAV preparations is essential for quality control in AAV production. Our AAV particles are tested for showing three clear bands of VP1, VP2 VP3 by SDS-PAGE.
Sterility	The AAV virus samples are inoculated into the cell culture medium for about 5 days to detect bacterial and fungal growth.
Transducibility	Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of AAV to deliver genetic material into target cells or tissues, and assess gene expression and functional activities.
Empty vs. Full Capsids	Based-on our proprietary AAV production and purification technology, Creative Biogene can always offer AAV particles with high ratio of full capsids. If required, we can also assess the ratio for a specifc lot of AAV particles by transmission electron microscopy (TEM) or other methods.

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Recombinant adeno-associated virus (rAAV) has become the most popular vector for gene therapy, which has transformed the treatment of harmful diseases that otherwise lack therapeutic options. Compared to other gene therapy delivery systems, AAV can effectively transduce both dividing and non-dividing cells, has low immunogenicity and provides long-term gene expression.

AAV consists of a capsid protein that encapsidates approximately 4.7 kb of single-stranded DNA (ssDNA). The capsid protein is assembled from 60 subunits, composed of VP1, VP2, and VP3 in a 1:1:10 ratio. Multiple naturally occurring AAV serotypes have been identified in humans and other primates, each of which exhibits different tissue and cell tropisms, transduction efficiency, and immunogenicity. Many currently available and emerging AAV gene therapies use these serotypes to target a wide range of diseases. AAV9 has demonstrated broad tissue tropism and enhanced transduction efficiency, acting on different organs, including the heart, skeletal muscle, liver, pancreas, and eyes. Furthermore, AAV9 has been shown to be able to cross the blood-brain barrier and target the central nervous system.

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Customer Reviews

Superior Quality

I am consistently impressed with the high quality of Creative Biogene’s AAV vectors. The packaging is exceptional, and the product arrives in perfect condition.

French

2024-12-27

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CAG-FLEX-jGCaMP7f AAV (Serotype 9)

Virus Particles Information

Quality Control

Background

Q & A

Customer Reviews

Superior Quality

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