CAG-Cre-GFP Lentivirus

Lentivirus is a type of retrovirus with a single-stranded RNA genome. However, unlike general retroviruses, lentiviruses have a wider host range and are able to infect both dividing and non-dividing cells. Recombinant lentivirus vectors are based on HIV-1 (human immunodeficiency virus type I) and are developed using the herpes virus VSVG coat protein. Their toxic genes have been removed and replaced by exogenous target genes, and they are pseudotyped viruses. Lentiviruses can effectively integrate exogenous genes into host chromosomes to achieve persistent expression. Lentiviruses can effectively infect various types of cells, such as neurons, hepatocytes, cardiomyocytes, tumor cells, endothelial cells, stem cells, etc., providing more powerful genetic research tools for researchers in the fields of neurology, cardiovascular, liver, muscle, eye, lung, tumor and other fields. Compared with other viral tools, lentivirus has the following advantages: a. Long expression time: By integrating exogenous genes into the host cell genome, lentivirus can achieve long-term stable expression of the target gene, which will not be lost with cell division and generation, and is the first choice for cell experiments; b. High safety: No pathogenicity has been found, and it has been used for CAR-T treatment in humans; c. Low immunogenicity: Direct injection of living tissue is not likely to cause immune response, and is suitable for animal experiments.