Cat.No. : AAV00294Z
Titer: ≥1x10^12 GC/mL / ≥1x10^13 GC/mL Size: 30 ul/100 ul/500 ul/1 ml
Serotype: AAV Serotype 9 Storage: -80 ℃
| Cat. No. | AAV00294Z |
| Description | AAV serotype 9 particles contain RFP under the Synapsin promoter. |
| Reporter | RFP |
| Serotype | AAV Serotype 9 |
| Application | 1. Determination of optimal MOI (multiplicity of infection), administration methods etc. 2. Detection of the infection efficiency of the AAV serotype against a specific cell type or tissue. 3. Using reporter genes to visualize the distribution and expression of AAV vectors in live animals, helping assess the biodistribution and persistence of gene delivery. |
| Titer | Varies lot by lot, typically ≥1x10^12 GC/mL |
| Size | Varies lot by lot, for example, 30 μL, 50 μL, 100 μL etc. |
| Storage | Store at -80℃. Avoid multiple freeze/thaw cycles. |
| Shipping | Frozen on dry ice |
| Creative Biogene ensures high-quality AAV particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between AAV particle lots. | |
| Endotoxin | Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in the production of AAV, especially for applications in animal studies and gene therapy. Effective endotoxin quality control is essential in the development and manufacturing of AAV particles. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced AAV particles to ensure regulatory compliance. |
| Purity | AAV purity is critical for ensuring the safety and efficacy of AAV-based applications.AAV capsids are composed of three main protein components, known as viral proteins: VP1, VP2, and VP3. These proteins play a critical role in the structure and functionality of the AAV capsid. Monitoring the VP1, VP2, and VP3 content in AAV preparations is essential for quality control in AAV production. Our AAV particles are tested for showing three clear bands of VP1, VP2 VP3 by SDS-PAGE. |
| Sterility | The AAV virus samples are inoculated into the cell culture medium for about 5 days to detect bacterial and fungal growth. |
| Transducibility | Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of AAV to deliver genetic material into target cells or tissues, and assess gene expression and functional activities. |
| Empty vs. Full Capsids | Based-on our proprietary AAV production and purification technology, Creative Biogene can always offer AAV particles with high ratio of full capsids. If required, we can also assess the ratio for a specifc lot of AAV particles by transmission electron microscopy (TEM) or other methods. |
Delicate interneuronal communication between presynaptic and postsynaptic membranes is essential for synaptic plasticity and memory formation. Evidence suggests that membrane/lipid rafts (MLRs), plasma membrane microdomains enriched with cholesterol and sphingolipids, organize presynaptic proteins and postsynaptic receptors essential for synapse formation and signaling. Here, researchers demonstrate that in the adult mouse hippocampus, neuronal-targeted overexpression of the MLR protein Caveolin-1 (SynCav1) increases the number of presynaptic vesicles per synaptic terminal, total excitatory type I glutamatergic synapses, the number of multisynaptic terminals on the same dendrite, increased myelination, increased long-term potentiation, and increased MLR-localized N-methyl-d-aspartate receptor subunits (GluN1, GluN2A, and GluN2B). Immunogold electron microscopy revealed that Cav-1 localizes to presynaptic and postsynaptic membrane regions as well as the synaptic cleft. These observations suggest that Cav-1 and MLRs alter fundamental aspects of synaptic biology and could serve as potential therapeutic targets for promoting neuroplasticity and combating neurodegeneration in a variety of neurological diseases.
Previous studies have shown that SynCav1 promotes dendritic arborization of hippocampal neurons and improves hippocampus-dependent learning and memory. Therefore, researchers performed electron microscopy (EM) on the hippocampus of adult mice injected with AAV9-Syn-RFP or AAV9-Syn-Cav1 to assess ultrastructural indicators of synaptic plasticity. SynCav1 significantly increased total excitatory synapses, had a clear asymmetry in postsynaptic synapse density, and significantly increased the number of PSVs and dsMSBs per axon terminal compared with mice receiving AAV9-Syn-RFP. The researchers also used G-ratio analysis to measure changes in myelination in the hippocampus of adult mice injected with AAV9-Syn-RFP or AAV9-Syn-Cav1. SynCav1 was found to significantly reduce the G ratio (Figure 1A). The decrease in G-ratio was not due to the narrowing of the axonal lumen (Figure 1B) but to an increase in myelin diameter (Figure 1C). These results indicate that SynCav1 affects ultrastructural changes in the hippocampal CA1 subfield consistent with those observed following events that promote synaptic plasticity.
Figure 1. SynCav1 increases myelin structure in the hippocampus. AAV9-Syn-RFP or AAV9-Syn-Cav1 was delivered directly to the hippocampus stereotaxically, and the brains were ready for conventional EM treatment 2 months later. (Egawa J, et al., 2018)
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The AAV9-Syn-RFP from Creative Biogene delivered consistent and reliable RFP expression under the human synapsin promoter. The pre-packaged format saved us significant time.
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