Cat.No. : AAV00262Z
Titer: ≥1x10^12 GC/mL / ≥1x10^13 GC/mL Size: 30 ul/100 ul/500 ul/1 ml
Serotype: AAV Serotype 9 Storage: -80 ℃
| Cat. No. | AAV00262Z |
| Description | AAV serotype 9 particles contain Cre recombinase fused with GFP under the human synapsin promoter. |
| Serotype | AAV Serotype 9 |
| Titer | Varies lot by lot, typically ≥1x10^12 GC/mL |
| Size | Varies lot by lot, for example, 30 μL, 50 μL, 100 μL etc. |
| Storage | Store at -80℃. Avoid multiple freeze/thaw cycles. |
| Shipping | Frozen on dry ice |
| Creative Biogene ensures high-quality AAV particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between AAV particle lots. | |
| Endotoxin | Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in the production of AAV, especially for applications in animal studies and gene therapy. Effective endotoxin quality control is essential in the development and manufacturing of AAV particles. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced AAV particles to ensure regulatory compliance. |
| Purity | AAV purity is critical for ensuring the safety and efficacy of AAV-based applications.AAV capsids are composed of three main protein components, known as viral proteins: VP1, VP2, and VP3. These proteins play a critical role in the structure and functionality of the AAV capsid. Monitoring the VP1, VP2, and VP3 content in AAV preparations is essential for quality control in AAV production. Our AAV particles are tested for showing three clear bands of VP1, VP2 VP3 by SDS-PAGE. |
| Sterility | The AAV virus samples are inoculated into the cell culture medium for about 5 days to detect bacterial and fungal growth. |
| Transducibility | Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of AAV to deliver genetic material into target cells or tissues, and assess gene expression and functional activities. |
| Empty vs. Full Capsids | Based-on our proprietary AAV production and purification technology, Creative Biogene can always offer AAV particles with high ratio of full capsids. If required, we can also assess the ratio for a specifc lot of AAV particles by transmission electron microscopy (TEM) or other methods. |
Recently, there has been a renewed interest in the therapeutic potential of serotonergic psychedelics. Here, researchers reveal an essential role for ventral hippocampal (vHpc) GABAergic interneurons in the anxiolytic effects induced by the serotonergic psychedelic 2,5-dimethoxy-4-iodoamphetamine (DOI). By integrating anatomical, pharmacological, and genetic approaches, studies show that 5-HT2A receptors in the CA1/subiculum (CA1/sub) region of the vHpc are required for the anxiolytic effects of DOI. In vivo electrophysiological and photolabeling experiments revealed that DOI increases the firing rate of fast-firing parvalbumin (PV)-positive interneurons in the hippocampus, most of which express 5-HT2A receptors. Restoration of 5-HT2A receptors in PV-positive interneurons in the context of loss-of-function rescued the anxiolytic responses induced by DOI in the vHpc CA1/sub region. Together, these findings localize the acute anxiolytic action of serotonergic psychedelics to 5-HT2A receptors in the ventral hippocampus and clearly identify PV-positive fast-spiking cells as the cellular triggers of psychedelic-induced relief of anxiety-like behaviors.
Here, researchers investigated whether restoring 5-HT2A receptor expression within the vHpc CA1/sub region in the 5-HT2ARKO mouse line is sufficient to result in anxiolytic behavioral responses on the EPM in response to acute, systemic DOI treatment. Using a viral delivery approach, infusion of AAV9-Syn-Cre-GFP into the vHpc CA1/sub region of the 5-HT2ARKO-resfl/fl mouse line restored neuronal 5-HT2A receptor expression in the vHpc CA1/sub region in a 5-HT2AR receptor loss-of-function background. Cre recombinase acts by excising a floxed neostop cassette located upstream of the htr2a gene, which helps conditionally restore 5-HT2A receptor expression in neurons expressing synapsin-Cre (Figures 1C and 1D). Researchers demonstrated that acute, systemic DOI administration in 5-HT2ARKO-resfl/fl mice, coupled with Cre recombinase-mediated rescue of 5-HT2A receptor expression in neurons within the vHpc CA1/sub region, resulted in a significant decrease in anxiety-like behaviors on the EPM, as observed by increased percentage time in the open arms and decreased percentage time in the closed arms ( Figures 1E-1G).
Figure 1. 5-HT2A receptors in the ventral hippocampal CA1/sub region mediate the decrease in anxiety-like behavior evoked by DOI. (Tiwari P, et al., 2024)
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Integrating AAV9-Syn-Cre-GFP into our mouse models was remarkably straightforward. It significantly cut down the setup time, allowing us to focus on data collection and analysis.
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