AAV9-CMV-saCas9-U6-sgRNA

Name: AAV9-CMV-saCas9-U6-sgRNA
SKU: AAV00264Z
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : AAV00264Z

Serotype : AAV Serotype 9 Storage : -80 ℃

Titer: Size:

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Virus Particles Information

Quality Control

Cat. No.	AAV00264Z
Description	AAV serotype 9 particles contain saCas9 nuclease under CMV promoter and scrambled sgRNA under U6 promoter.
Serotype	AAV Serotype 9
Titer	Varies lot by lot, typically ≥1x10^12 GC/mL
Size	Varies lot by lot, for example, 30 μL, 100 μL, 500 μL etc.
Storage	Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping	Frozen on dry ice

Summary	Creative Biogene ensures high-quality AAV particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between AAV particle lots.
Endotoxin	Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in the production of AAV, especially for applications in animal studies and gene therapy. Effective endotoxin quality control is essential in the development and manufacturing of AAV particles. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced AAV particles to ensure regulatory compliance.
Purity	AAV purity is critical for ensuring the safety and efficacy of AAV-based applications.AAV capsids are composed of three main protein components, known as viral proteins: VP1, VP2, and VP3. These proteins play a critical role in the structure and functionality of the AAV capsid. Monitoring the VP1, VP2, and VP3 content in AAV preparations is essential for quality control in AAV production. Our AAV particles are tested for showing three clear bands of VP1, VP2 VP3 by SDS-PAGE.
Sterility	The AAV virus samples are inoculated into the cell culture medium for about 5 days to detect bacterial and fungal growth.
Transducibility	Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of AAV to deliver genetic material into target cells or tissues, and assess gene expression and functional activities.
Empty vs. Full Capsids	Based-on our proprietary AAV production and purification technology, Creative Biogene can always offer AAV particles with high ratio of full capsids. If required, we can also assess the ratio for a specifc lot of AAV particles by transmission electron microscopy (TEM) or other methods.

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AAV is a non-enveloped virus that can be engineered to deliver DNA to target cells. The viral genome is not integrated into the host cell, but rather forms episomal concatemers in the host cell nucleus. These head-to-tail circular concatemers remain intact in non-dividing cells, such as neurons and cardiomyocytes, and are therefore able to express transgenes for months. AAV vectors also provide relatively stable expression in dividing cells. The frequency of integration events may increase if very high multiplicities of infection are used or if cells are infected in the presence of adenoviral replicase.

AAV vectors are often the first choice for in vivo gene therapy because of their multiple advantages, including the ability to transduce both dividing and quiescent cells, strong in vivo transduction efficiency, long-term transgene expression in quiescent cells, tropism for specific tissues and cell types, relatively low immunogenicity, lack of pathogenicity, and a history of clinical safety.

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Customer Reviews

Excellent Customer Support

On several occasions, I reached out to the customer support team for assistance with the AAV9-CMV-saCas9-U6-sgRNA product, and each interaction was met with prompt and helpful responses. Their expertise and willingness to assist have greatly facilitated the troubleshooting process, enhancing our overall experience with the product.

United Kingdom

2022-07-18

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AAV9-CMV-saCas9-U6-sgRNA

Virus Particles Information

Quality Control

Background

Q & A

Customer Reviews

Excellent Customer Support

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