Cat.No. : AAV00213Z
Titer: ≥1x10^12 GC/mL / ≥1x10^13 GC/mL Size: 30 ul/100 ul/500 ul/1 ml
Serotype: AAV Serotype 8 Storage: -80 ℃
| Cat. No. | AAV00213Z |
| Description | AAV serotype 8 particles contain mCherry under CMV promoter. |
| Reporter | mCherry |
| Serotype | AAV Serotype 8 |
| Application | 1. Determination of optimal MOI (multiplicity of infection), administration methods etc. 2. Detection of the infection efficiency of the AAV serotype against a specific cell type or tissue. 3. Using reporter genes to visualize the distribution and expression of AAV vectors in live animals, helping assess the biodistribution and persistence of gene delivery. |
| Titer | Varies lot by lot, typically ≥1x10^12 GC/mL |
| Size | Varies lot by lot, for example, 30 μL, 50 μL, 100 μL etc. |
| Storage | Store at -80℃. Avoid multiple freeze/thaw cycles. |
| Shipping | Frozen on dry ice |
| Creative Biogene ensures high-quality AAV particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between AAV particle lots. | |
| Endotoxin | Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in the production of AAV, especially for applications in animal studies and gene therapy. Effective endotoxin quality control is essential in the development and manufacturing of AAV particles. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced AAV particles to ensure regulatory compliance. |
| Purity | AAV purity is critical for ensuring the safety and efficacy of AAV-based applications.AAV capsids are composed of three main protein components, known as viral proteins: VP1, VP2, and VP3. These proteins play a critical role in the structure and functionality of the AAV capsid. Monitoring the VP1, VP2, and VP3 content in AAV preparations is essential for quality control in AAV production. Our AAV particles are tested for showing three clear bands of VP1, VP2 VP3 by SDS-PAGE. |
| Sterility | The AAV virus samples are inoculated into the cell culture medium for about 5 days to detect bacterial and fungal growth. |
| Transducibility | Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of AAV to deliver genetic material into target cells or tissues, and assess gene expression and functional activities. |
| Empty vs. Full Capsids | Based-on our proprietary AAV production and purification technology, Creative Biogene can always offer AAV particles with high ratio of full capsids. If required, we can also assess the ratio for a specifc lot of AAV particles by transmission electron microscopy (TEM) or other methods. |
Genetic mutations cause testosterone deficiency and infertility. Serum testosterone levels can be restored by testosterone replacement therapy. However, existing therapies have limited efficacy in restoring fertility. Here, researchers used a luteinizing hormone/chorionic gonadotropin receptor (Lhcgr)-deficient LCF mouse model to investigate the feasibility of gene therapy to restore testosterone production and fertility. They screened several adeno-associated virus (AAV) serotypes and identified AAV8 as an efficient vector to drive exogenous Lhcgr expression in progenitor Leydig cells via interstitial injection. Significant restoration of testosterone and Leydig cell maturation was observed in adolescent Lhcgr−/− mice treated with AAV8-Lhcgr. Notably, this gene therapy partially restored sexual development, significantly restored spermatogenesis, and efficiently produced fertile offspring. Furthermore, these favorable effects could be recapitulated in adult Lhcgr−/− mice.
Here, researchers injected a commonly used AAV8-mCherry vector at a titer of 8 X 1010 genome copies (gc) into each testis of Lhcgr−/− mice. Immunofluorescence analysis showed that mCherry was expressed in the testis, but not in the liver, heart, muscle, kidney, or colon (Figure 1A), indicating that AAV8 exhibited a clear testicular tropism when injected into the testis interstitially. Quantitative RT-PCR analysis of testicular tissue showed that injection of AAV8-mCherry resulted in a transient increase in transcripts of key inflammatory genes in the testis on days 1 and 3, while the expression levels of these genes returned to normal levels within 7 days (Figure 1B). Further examination of the infiltration of CD4+ and CD8+ lymphocytes 7 days after AAV8-mCherry injection showed no significant difference in lymphocyte infiltration between the injected and uninjected groups (Figure 1C). These results indicate that interstitial injection of AAV8 is safe and well tolerated.
Figure 1. AAV8 showed a clear testis tropism and safety profile with no apparent inflammatory reaction. (Xia K, et al., 2022)
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We used AAV8-mCherry for in vivo studies and the transduction efficiency was excellent. The fluorescence intensity was strong and consistent across our samples, making it easy to track gene expression.
As a researcher with limited experience in viral vectors, I found AAV8-mCherry to be incredibly user-friendly.
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