AAV8-CB6-GFP

Name: AAV8-CB6-GFP
SKU: AAV00228Z
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : AAV00228Z

Serotype : AAV Serotype 8 Storage : -80 ℃

Titer: Size:

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Cat. No.	AAV00228Z
Description	AAV serotype 8 particles contain GFP under a hybrid CB6 promoter.
Serotype	AAV Serotype 8
Reporter	GFP
Applications	1. Determination of optimal MOI (multiplicity of infection), administration methods etc. 2. Detection of the infection efficiency of the AAV serotype against a specific cell type or tissue. 3. Using reporter genes to visualize the distribution and expression of AAV vectors in live animals, helping assess the biodistribution and persistence of gene delivery.
Titer	Varies lot by lot, typically ≥1x10^12 GC/mL
Size	Varies lot by lot, for example, 30 μL, 100 μL, 500 μL etc.
Storage	Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping	Frozen on dry ice

Summary	Creative Biogene ensures high-quality AAV particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between AAV particle lots.
Endotoxin	Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in the production of AAV, especially for applications in animal studies and gene therapy. Effective endotoxin quality control is essential in the development and manufacturing of AAV particles. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced AAV particles to ensure regulatory compliance.
Purity	AAV purity is critical for ensuring the safety and efficacy of AAV-based applications.AAV capsids are composed of three main protein components, known as viral proteins: VP1, VP2, and VP3. These proteins play a critical role in the structure and functionality of the AAV capsid. Monitoring the VP1, VP2, and VP3 content in AAV preparations is essential for quality control in AAV production. Our AAV particles are tested for showing three clear bands of VP1, VP2 VP3 by SDS-PAGE.
Sterility	The AAV virus samples are inoculated into the cell culture medium for about 5 days to detect bacterial and fungal growth.
Transducibility	Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of AAV to deliver genetic material into target cells or tissues, and assess gene expression and functional activities.
Empty vs. Full Capsids	Based-on our proprietary AAV production and purification technology, Creative Biogene can always offer AAV particles with high ratio of full capsids. If required, we can also assess the ratio for a specifc lot of AAV particles by transmission electron microscopy (TEM) or other methods.

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Adeno-associated virus (AAV) is a single-stranded DNA virus that is not currently associated with human diseases. In recent years, after decades of unremitting efforts, the field of gene therapy based on AAV vectors has achieved several successes. The AAV1-LDL vector for the treatment of lipoprotein lipase deficiency was approved in Europe in 2012. In addition, clinical trials using AAV vectors to treat Leber's congenital amaurosis 2 and hemophilia B3 have reported good results.

AAV belongs to the family Parvoviridae and is a small (about 20 nanometers), non-pathogenic, non-enveloped, icosahedral virus that packages 4.8 kb ssDNA. Their genome consists of two major genes, rep and cap, flanked by T-shaped hairpin structures called inverted terminal repeats (ITRs). In addition to its complex structure, AAV also exhibits differences between serotypes, as well as between and within batches. This variability includes differences in post-translational modifications (PTMs), capsid stoichiometry, and encapsidated genome sequences.

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Customer Reviews

Exceptional Product Quality

We were thoroughly impressed with the purity and quality of the AAV8-CB6-GFP vector. It consistently showed high transduction efficiency in our experiments, which greatly contributed to the success of our research project.

French

2020-04-28

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AAV8-CB6-GFP

Virus Particles Information

Quality Control

Background

Q & A

Customer Reviews

Exceptional Product Quality

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