Transfected Stable Cell Lines
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Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : AAV00162Z
Serotype : AAV Serotype 2 Storage : -80 ℃
Titer: Size:
| Cat. No. | AAV00162Z |
| Description | AAV serotype 2 particles contain codon-improved Cre (iCre) under CAG promoter. |
| Serotype | AAV Serotype 2 |
| Titer | Varies lot by lot, typically ≥1x10^12 GC/mL |
| Size | Varies lot by lot, for example, 30 μL, 100 μL, 500 μL etc. |
| Storage | Store at -80℃. Avoid multiple freeze/thaw cycles. |
| Shipping | Frozen on dry ice |
| Summary | Creative Biogene ensures high-quality AAV particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between AAV particle lots. |
| Endotoxin | Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in the production of AAV, especially for applications in animal studies and gene therapy. Effective endotoxin quality control is essential in the development and manufacturing of AAV particles. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced AAV particles to ensure regulatory compliance. |
| Purity | AAV purity is critical for ensuring the safety and efficacy of AAV-based applications.AAV capsids are composed of three main protein components, known as viral proteins: VP1, VP2, and VP3. These proteins play a critical role in the structure and functionality of the AAV capsid. Monitoring the VP1, VP2, and VP3 content in AAV preparations is essential for quality control in AAV production. Our AAV particles are tested for showing three clear bands of VP1, VP2 VP3 by SDS-PAGE. |
| Sterility | The AAV virus samples are inoculated into the cell culture medium for about 5 days to detect bacterial and fungal growth. |
| Transducibility | Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of AAV to deliver genetic material into target cells or tissues, and assess gene expression and functional activities. |
| Empty vs. Full Capsids | Based-on our proprietary AAV production and purification technology, Creative Biogene can always offer AAV particles with high ratio of full capsids. If required, we can also assess the ratio for a specifc lot of AAV particles by transmission electron microscopy (TEM) or other methods. |
Adeno-associated viruses (AAV) belong to the family Parvoviridae. These viruses belong to the genus Dependovirus, whose members require a helper virus (such as adenovirus or herpes simplex virus) to facilitate productive infection and replication. In the absence of a helper virus, AAV establishes a latent infection within the cell, either by site-specific integration into the host genome or by persisting in an episomal form.
The virion capsid is approximately 25 nm in diameter and encapsulates a 4.7 kb single-stranded DNA genome composed of two large open reading frames (ORFs) flanked by inverted terminal repeats (ITRs). The ITRs are the only cis-acting elements required for genome replication and packaging. The left ORF encodes four replication proteins responsible for site-specific integration, nicking and helicase activity, and regulation of promoters within the AAV genome. The right ORF encodes the viral structural proteins VP1, VP2, and VP3, which assemble into the icosahedral virion capsid, each composed of 60 subunits.
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We were impressed with the high efficiency of AAV2-CAG-iCre in our project. The viral delivery was consistent, and the expression levels were robust, leading to successful recombination in our target cells.
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