Cat.No. : AAV00097Z
Titer: ≥1x10^12 GC/mL / ≥1x10^13 GC/mL Size: 30 ul/100 ul/500 ul/1 ml
Serotype: AAV Serotype 8 Storage: -80 ℃
| Cat. No. | AAV00097Z |
| Description | AAV (Serotype 8) is the serotype 8 AAV with CMV promoter with no insert gene. Used as a control. |
| Serotype | AAV Serotype 8 |
| Product Type | Adeno-associated virus particles |
| Promoter | CMV |
| Titer | Varies lot by lot, typically ≥1x10^12 GC/mL |
| Size | Varies lot by lot, for example, 30 μL, 50 μL, 100 μL etc. |
| Storage | Store at -80℃. Avoid multiple freeze/thaw cycles. |
| Shipping | Frozen on dry ice |
| Creative Biogene ensures high-quality AAV particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between AAV particle lots. | |
| Endotoxin | Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in the production of AAV, especially for applications in animal studies and gene therapy. Effective endotoxin quality control is essential in the development and manufacturing of AAV particles. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced AAV particles to ensure regulatory compliance. |
| Purity | AAV purity is critical for ensuring the safety and efficacy of AAV-based applications.AAV capsids are composed of three main protein components, known as viral proteins: VP1, VP2, and VP3. These proteins play a critical role in the structure and functionality of the AAV capsid. Monitoring the VP1, VP2, and VP3 content in AAV preparations is essential for quality control in AAV production. Our AAV particles are tested for showing three clear bands of VP1, VP2 VP3 by SDS-PAGE. |
| Sterility | The AAV virus samples are inoculated into the cell culture medium for about 5 days to detect bacterial and fungal growth. |
| Transducibility | Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of AAV to deliver genetic material into target cells or tissues, and assess gene expression and functional activities. |
| Empty vs. Full Capsids | Based-on our proprietary AAV production and purification technology, Creative Biogene can always offer AAV particles with high ratio of full capsids. If required, we can also assess the ratio for a specifc lot of AAV particles by transmission electron microscopy (TEM) or other methods. |
Alcohol-related liver disease (ALD) is the leading cause of alcohol-related mortality. Sex is an important variable; however, the mechanisms underlying sex differences have not been identified. Here, researchers found that Kdm5b/Kdm5c knockout promoted alcohol-induced liver disease, whereas gonadectomy abolished this effect, suggesting that male sex hormones promote liver disease in the absence of KDM5 demethylase. In contrast, in a thioacetamide-induced fibrosis model, male sex hormones showed a protective effect regardless of genotype. In human liver disease samples, androgen receptor expression was found to be positively correlated with fibrosis levels when KDM5B levels were low, while it was negatively correlated with fibrosis levels when KDM5B levels were high, suggesting that KDM5B-dependent epigenetic states determine the role of androgen receptor in liver fibrosis. In KDM5-deficient mice, Notch3 and Jag1 gene expression was induced, promoting testosterone-mediated Notch signaling and stellate cell activation. Inhibition of Notch using avagacestat significantly reduced liver fibrosis and abolished the effects of Kdm5b/Kdm5c loss.
Here, the researchers used Kdm5b flox/flox and Kdm5b flox/flox Kdm5c flox male mice, which were subjected to GDX or sham surgery. After 2 weeks of recovery, mice were fed Western diet (WD) with 20% alcohol (WDA) in the drinking water for 18 weeks. Two weeks after the start of drinking, mice received 2 X 1011 AAV8-CMV-Cre (KO) or AAV8-control (WT) genome copies. Kdm5b KO resulted in a dramatic decrease in KDM5B and KDM5C protein levels in hepatocytes and non-parenchymal cells in the liver (Figure 1A). Although Kdm5b KO itself had no obvious phenotype, researchers noticed that Kdm5b and Kdm5c KO in sham-operated mice resulted in reduced weight gain (Figure 1B), and the mice looked sicker compared to all other groups. Interestingly, food intake was increased in sham-operated KO mice (Figure 1C). Mice receiving GDX also exhibited reduced weight gain, which correlated with reduced food intake (Figure 1B, C). Furthermore, in GDX mice, the researchers observed no differences in weight gain or food intake between wild-type (WT) and KO mice. Alcohol intake was similar in all 4 groups (Figure 1D).
Figure 1. Kdm5b and Kdm5c KO promotes ALD in a male sex hormone-dependent way. (Nataraj K, et al., 2024)
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As a control vector with no insert gene, the AAV Serotype 8 has been essential in calibrating our experiments.
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