AAV PHP.eB-CAG-Cre

Adeno-associated viral (AAV) vectors are small, non-enveloped, non-pathogenic, single-stranded DNA parvoviruses that infect a variety of cell types, including many types of neurons and glial cells. Thirteen naturally occurring AAV serotypes of human and simian origin have been reported, which are recognized by different cell surface receptors and have different cellular affinities. For example, astrocytes are the predominant cell type transduced by AAV serotype 8, whereas AAV serotype 9 (AAV9) primarily transduces neurons. Deverman et al. reported AAV-PHP.B (hereinafter referred to as PHP.B), which is a capsid variant of AAV9 with 7 amino acids inserted, showing an extremely high ability to penetrate the mouse blood-brain barrier (BBB). . Intravenous infusion of PHP.B effectively transduces the entire brain. In addition, the research team also modified AAV-PHP.eB (hereinafter referred to as PHP.eB), which differs from PHP.B by only 2 amino acids near the heptamer insertion site. After intravenous injection of PHP.eB, the transduction effect on cerebral cortex neurons was significantly enhanced, and the transduction efficiency was higher than that of PHP.B.