Panoply™ Human SMO Over-expressing Stable Cell Line

Name: Panoply™ Human SMO Over-expressing Stable Cell Line
SKU: CSC-SC014764
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC014764

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

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Gene Information

Cat. No.	CSC-SC014764
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	SMO
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	SMO smoothened, frizzled family receptor [ Homo sapiens ]
Gene Symbol	SMO
Synonyms	Gx; SMOH; FZD11
Gene ID	6608
Uni Prot ID	A4D1K5
m RNA Refseq	NM_005631.4
Protein Refseq	NP_005622.1
Chromosome Location	7q32.3
Function	G-protein coupled receptor activity; PDZ domain binding; Wnt-activated receptor activity; Wnt-protein binding; drug binding; patched binding; protein binding;
Pathway	Basal cell carcinoma, organism-specific biosystem; Basal cell carcinoma, conserved biosystem; Class B/2 (Secretin family receptors), organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; GPCRs, Other, organism-specific biosystem; Hedgehog Signaling Pathway, organism-specific biosystem; Hedgehog signaling events mediated by Gli proteins, organism-specific biosystem;
MIM	601500

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Glioblastoma (GBM) is one of the most aggressive and lethal types of human cancer. Standard treatment involves surgery followed by combined chemoradiation. However, the molecular mechanisms underlying innate tumor radioresistance remain unclear. Here, researchers found that Smoothened (Smo) expression is significantly upregulated in recurrent GBM cell lines and tumor tissues after radiotherapy. Increased Smo expression is associated with a poor prognosis in GBM patients treated with radiotherapy. Smo conferred radioresistance in both GBM cells and human tumor xenografts. The underlying mechanism of these effects involves impaired repair of radiotherapy-induced DNA damage. Importantly, Smo's effect on radioresistance is mediated by claspin polyubiquitination and proteasomal degradation, thereby regulating ATR-Chk1 signaling. Furthermore, Smo reduces claspin polyubiquitination and proteasomal degradation by promoting USP3 transcription. Researchers also demonstrated that the Smo inhibitor GDC-0449 induces radiosensitivity in GBM. These findings reveal the underlying mechanism of GBM radioresistance and suggest a potential therapeutic target for radiation resistance in GBM.

To investigate whether Smo is associated with radioresistance, the researchers generated Smo-overexpressing primary GBM cells (survival rate less than 45% after 2 Gy irradiation). As expected, Smo was overexpressed in both primary GBM cell lines (Figure 1A). They then investigated the effect of Smo overexpression on the survival of primary GBM cells after irradiation (IR). As shown in Figure 1B, Smo overexpression enhanced the resistance of primary GBM cells to IR. Next, they assessed the apoptosis rate of Smo-overexpressing and control cells after IR. The results showed that compared with control cells, Smo-overexpressing cells had significantly fewer apoptotic cells (Annexin V-PI/FITC or TUNEL staining) and significantly decreased levels of cleaved caspase-3 (Figures 1C-E). These results indicate that Smo overexpression induces resistance to IR in primary GBM cells and protects them from IR-induced apoptosis.

Figure 1. Smo overexpression confers radiation resistance. (Tu Y, et al., 2020)

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