Cat.No. : CSC-DC011260
Host Cell: HEK293 (Hela and other cell types are also available) Validation: Real-Time RCR
| Cat. No. | CSC-DC011260 |
| Description | Creative Biogene's Knockdown Cell Lines are target specific shRNA lentivirus transduced cells. The percent knockdown levels range from 75-99% depending on the gene, as evaluated by Real-Time RCR. Cells are rigorously qualified and mycoplasma free. |
| Gene | PAK1 |
| Host Cell | HEK293 (Hela and other cell types are also available) |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | (1) Studying gene functions (2) Studying gene interactions and signaling pathways (3) Target validation and drug discovery (4) Designing diseases models |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Size Form | >1 × 10^6 cells / vial |
| Shipping | Dry Ice |
| Storage | Liquid Nitrogen |
| Gene Name | PAK1 p21 protein (Cdc42/Rac)-activated kinase 1 [ Homo sapiens ] |
| Gene Symbol | PAK1 |
| Synonyms | PAK1; p21 protein (Cdc42/Rac)-activated kinase 1; p21/Cdc42/Rac1 activated kinase 1 (STE20 homolog, yeast) , p21/Cdc42/Rac1 activated kinase 1 (yeast Ste20 related); serine/threonine-protein kinase PAK 1; STE20 homolog; yeast; p65-PAK; alpha-PAK; STE20 homolog, yeast; p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related); p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast); PAKalpha; MGC130000; MGC130001; |
| Gene ID | 5058 |
| UniProt ID | Q13153 |
| mRNA Refseq | BC109299 |
| Chromosome Location | 11q13-q14 |
| Function | ATP binding; collagen binding; nucleotide binding; protein binding; contributes_to protein binding; |
| Pathway | Activation of Rac, organism-specific biosystem; Adaptive Immune System, organism-specific biosystem; Alpha6-Beta4 Integrin Signaling Pathway, organism-specific biosystem; Angiopoietin receptor Tie2-mediated signaling, organism-specific biosystem; Aurora A signaling, organism-specific biosystem; Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem; |
| MIM | 602590 |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-related death. PAK1 plays a crucial role in various cancers. However, the role of PAK1 in HCC remains unclear. Here, researchers show that both PAK1 and Snail are upregulated in HCC cells. Knockdown of PAK1 inhibits the proliferation, migration, and invasion of HCC cells and promotes apoptosis. PAK1 knockdown also inhibits tumor growth in vivo. Mechanistically, PAK1 promotes the EMT process by targeting Snail. PAK1 knockdown inhibits the β-catenin signaling pathway, upregulates the expression of pro-apoptotic proteins, and downregulates the expression of proliferation-related proteins. Therefore, PAK1 promotes the EMT process by increasing Snail expression and promotes HCC progression by activating the β-catenin pathway.
To further determine the role of PAK1 in HCC, researchers investigated how PAK1 levels affect the survival of HCC cells. Through colony formation assays, researchers found that the number of colonies formed by PAK1-knockdown HCC cells was significantly reduced (Figure 1A-B), indicating that shPAK1 inhibited liver cancer cell proliferation. Furthermore, compared to the control group, apoptosis was significantly increased in PAK1-knockdown HCC cells. In SMC-7721 and Bel-7402 cells, PAK1 knockdown increased the apoptosis rate from 1.97% to 21.39% and from 1.83% to 28.24%, respectively (Figure 1C-D). Therefore, inhibiting PAK1 can suppress the proliferation of liver cancer cells while promoting their apoptosis.
Figure 1. Knockdown of PAK1 inhibited proliferation but promoted apoptotic death of HCC cells. (Cao F, Yin L X., 2020)
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