Panoply™ Human MUC16 Knockdown Stable Cell Line

Name: Panoply™ Human MUC16 Knockdown Stable Cell Line
SKU: CSC-DC009930
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-DC009930

Host Cell : HEK293 (Hela and other cell types are also available) Validation : Real-Time RCR

Inquire for Price

Cell Line Information

Safety and Packaging

Gene Information

Cat. No.	CSC-DC009930
Description	Creative Biogene's Knockdown Cell Lines are target specific shRNA lentivirus transduced cells. The percent knockdown levels range from 75-99% depending on the gene, as evaluated by Real-Time RCR. Cells are rigorously qualified and mycoplasma free.
Target Gene	MUC16
Host Cell	HEK293 (Hela and other cell types are also available)
Host Cell Species	Homo sapiens (Human)
Applications	(1) Studying gene functions (2) Studying gene interactions and signaling pathways (3) Target validation and drug discovery (4) Designing diseases models
Size	>1 × 10⁶ cells / vial
Stability	Validated for at least 10 passages
Validation	Real-Time RCR
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid Nitrogen
Shipping	Dry Ice

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	MUC16 mucin 16, cell surface associated [ Homo sapiens ]
Gene Symbol	MUC16
Synonyms	CA125
GeneID	94025
Uni ProtID	B3KY81
mRNA Refseq	NM_024690.2
Protein Refseq	NP_078966.2
Chromosome Location	19p13.2
Function	protein binding;
Pathway	Metabolism of proteins, organism-specific biosystem; O-linked glycosylation of mucins, organism-specific biosystem; Post-translational protein modification, organism-specific biosystem; Termination of O-glycan biosynthesis, organism-specific biosystem;
MIM	606154

Quick Inquiry

Case Study

Q & A

Customer Reviews

MUC16/CA125 has been clinically validated as an important tumor biomarker, particularly in epithelial ovarian cancer, for over three decades. Due to its large molecular weight, previous research on it has been limited. Here, researchers, utilizing a previously characterized, in-house monoclonal antibody against MUC16, identified for the first time an endogenous interaction between the C-terminal fragment of MUC16 (MUC16C) and β-catenin, further elucidating that this interaction requires the transactivation domain of β-catenin. This interaction activates the Wnt/β-catenin signaling pathway by promoting cytoplasmic-nuclear translocation of β-catenin, thereby inducing cell proliferation and migration, ultimately leading to tumor formation and metastasis in nude mice. Knockdown of the MUC16 gene significantly reduced cell proliferation and migration. Therefore, MUC16 represents an attractive target for the development of effective anticancer drugs.

Studies have shown that decreased MUC16 mRNA levels are associated with decreased mRNA levels of Wnt downstream genes (Cyclin D1, Axin2, c-Myc, Snail, and Survivin) and two EMT markers (Vimentin and Fibronectin), while increased mRNA levels of another EMT marker, E-cadherin (Figure 1A). Furthermore, in MUC16-knockdown SKBR-3 cells, protein levels of three Wnt downstream genes, Cyclin D1, c-Myc, and Axin2, were also decreased, as confirmed by two independent short-chain RNAs targeting MUC16 (Figure 1B). CCK8 assays demonstrated that MUC16 knockdown inhibited SKBR-3 cell proliferation (Figure 1C). This finding was supported by results from a colony formation assay: compared with control cells, MUC16-knockdown SKBR-3 cells exhibited reduced colony formation (Figure 1D). Furthermore, knockdown of MUC16 in SKBR-3 cells inhibited their migration and invasion abilities (Figure 1E). Therefore, MUC16 gene knockdown can reduce cell proliferation and migration ability.

Figure 1. MUC16 knockdown reduces proliferation and metastasis of SKBR-3 cells. (Liu Q, et al., 2016)

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Question *

Name

Country *

Email *

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Product Name *

Catalog Name *

Rating

Needs improvement

Satisfaction

General satisfaction

Very satisfaction

Product Review Title *

Summary *