Panoply™ Human GNRHR Over-expressing Stable Cell Line

Name: Panoply™ Human GNRHR Over-expressing Stable Cell Line
SKU: CSC-SC006427
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC006427

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

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Gene Information

Cat. No.	CSC-SC006427
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	GNRHR
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	GNRHR gonadotropin-releasing hormone receptor [ Homo sapiens ]
Gene Symbol	GNRHR
Synonyms	HH7; GRHR; LRHR; LHRHR; GNRHR1
Gene Description	gonadotropin-releasing hormone receptor
GeneID	2798
Uni ProtID	P30968
mRNA Refseq	NM_000406.2
Protein Refseq	NP_000397.1
Chromosome Location	4q21.2
Function	gonadotropin-releasing hormone receptor activity; growth hormone-releasing hormone receptor activity;
Pathway	Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; G alpha (q) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; GPCRs, Other, organism-specific biosystem; Gastrin-CREB signalling pathway via PKC and MAPK, organism-specific biosystem; GnRH signaling pathway, organism-specific biosystem;
MIM	138850

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Cancer is a leading cause of death worldwide, particularly reproductive-related cancers (such as breast/cervical cancer in women and prostate cancer in men). Chemotherapy is the most common systemic cancer therapy, but its side effects are concerning. Developing effective novel anticancer strategies is crucial to improving efficacy and minimizing side effects. Here, researchers used high-throughput microfluidics to fabricate novel core-shell lipid nanoparticles (LNPs) for chemotherapy drug delivery. Gonadotropin-releasing hormone (GnRH), a molecule that targets the gonadotropin-releasing hormone receptor (GnRHR) overexpressed in cancer cells, was conjugated to chitosan (GnRH-CS) as a shell and used to modify the LNP surface for doxorubicin loading, forming an LNP-dox-GnRH complex. The modified surface enhanced the binding affinity of the LNP for breast and prostate cancer cells. In in vitro studies, the researchers demonstrated that LNP-dox-GnRH specifically targeted GnRHR-overexpressing cancer cells (such as MCF-7 and PC-3 cells) compared to non-targeted LNP-dox. In contrast, there was no difference in targeting between LNP-dox-GnRHR and non-targeted LNPs to the GnRHR-low-expressing cancer cell MDA-MB-436. Furthermore, enhanced anticancer activity of LNP-dox-GnRH was observed in both monolayer and spheroid cell cultures.

LNP-dox-GnRH and doxorubicin (free drug) exhibited anticancer activity against the proliferation of GnRHR-overexpressing cancer cells, including hormone-dependent MCF-7 and PC-3 cells, while MDA-MB-436 cells are hormone-independent. Using doxorubicin (free drug) as a control, the cytotoxic effect of LNP-dox-GnRH was demonstrated to be attributable to its surface targeting moiety. The results indicated that the efficacy of LNP-dox-GnRH in inducing cell death in GnRHR overexpressing MCF-7 cells and PC-3 cells was considerably superior to the cell death obtained from the free doxorubicin and LNP-dox treated groups, particularly in the case of the MCF-7 cells. In contrast, in MDA-MB-436 cells, no difference in cytotoxicity was observed between LNP-dox and LNP-dox-GnRH-treated groups, attributed to lower GnRH receptor expression (Figure 1). Based on these findings, the anticancer effect of the LNP-dox-GnRH-treated group may be enhanced compared with LNP-dox and free DOX (absorbed by passive diffusion) because LNP-dox-GnRH is more significantly internalized via receptor-mediated endocytosis.

Figure 1. Cell viability of MCF-7 (A), PC-3 (B), and MDA-MB-436 (C) after LNP-dox-GnRH, LNP-dox, and doxorubicin (free drug) treatments. (Bunwatcharaphansakun P, et al., 2025)

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