Cat.No. : CSC-SC002868 Host Cell: HEK293 (CHO and other cell types are also available)
| Cat. No. | CSC-SC002868 |
| Description | Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level. |
| Gene | CDK19 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293 (CHO and other cell types are also available) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Size Form | 2 × 10^6 cells / vial |
| Shipping | Dry Ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Gene Name | CDK19 cyclin-dependent kinase 19 [ Homo sapiens ] |
| Gene Symbol | CDC2L6 |
| Synonyms | CDK11; CDC2L6; bA346C16.3 |
| Gene Description | cell division cycle 2-like 6 (CDK8-like) |
| Gene ID | 23097 |
| UniProt ID | Q9BWU1 |
| mRNA Refseq | NM_015076.3 |
| Protein Refseq | NP_055891.1 |
| Chromosome Location | 6q21 |
| Function | ATP binding; cyclin-dependent protein kinase activity; |
| Pathway | Developmental Biology, organism-specific biosystem; Fatty acid, triacylglycerol, and ketone body metabolism, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of lipids and lipoproteins, organism-specific biosystem; PPARA Activates Gene Expression, organism-specific biosystem; Regulation of Lipid Metabolism by Peroxisome proliferator-activated receptor alpha (PPARalpha), organism-specific biosystem; Transcriptional Regulation of White Adipocyte Differentiation, organism-specific biosystem; |
| MIM | 614720 |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Previous studies have shown that miR-222-3p is a tumor-suppressive miRNA in ovarian cancer (OC). Here, researchers aimed to further understand the regulatory role of miR-222-3p in ovarian cancer and provide new mechanisms for its prevention and treatment. The researchers found that miR-222-3p inhibits the migration and proliferation of ovarian cancer cells. CDK19 is highly expressed in ovarian cancer and is negatively correlated with miR-222-3p. Furthermore, miR-222-3p directly binds to the 3′-UTR of CDK19 and inhibits its translation, thereby inhibiting the migration and proliferation of ovarian cancer cells in vitro and inhibiting tumor growth in vivo. They also observed that Hotair inhibits miR-222-3p in ovarian cancer. In addition, Hotair can promote the proliferation and migration of ovarian cancer cells in vitro and promote tumor growth and metastasis in vivo. Moreover, the expression of Hotair is positively correlated with the expression of CDK19. These results indicate that Hotair indirectly upregulates the expression of CDK19 by "sponging" miR-222-3p, thereby enhancing the malignant behavior of ovarian cancer.
Here, researchers investigated the function of CDK19. Both colony formation assays (Figure 1I) and EdU assays showed a significant increase in cell proliferation in CDK19 overexpressing OVCAR3 cells. To determine whether miR-222-3p inhibits ovarian cancer cell proliferation by targeting CDK19, the researchers designed the following rescue experiment. In both colony formation assays and EdU assays, overexpression of CDK19 abolished the inhibitory effect of miR-222-3p mimics on OVCAR3 cell proliferation (Figure 1J, K). As expected, colony formation assays and flow cytometry experiments showed that overexpression of CDK19 could also abolish the inhibitory effect of miR-222-3p mimics on HO-8910 PM cell proliferation. Furthermore, compared to the control group, the group co-transfected with miR-ctrl mimics and CDK19 showed higher levels of CDK19 mRNA and protein. Similarly, the CDK19 mRNA and protein levels, which were suppressed by miR-222-3p mimics, were effectively restored in CDK19-overexpressing HO-8910 PM cells.
Figure 1. miR-222-3p inhibits ovarian cancer cell proliferation by targeting CDK19. (Fan L, et al., 2022)
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