Panoply™ Human CDK19 Over-expressing Stable Cell Line

Name: Panoply™ Human CDK19 Over-expressing Stable Cell Line
SKU: CSC-SC002868
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC002868

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

Inquire for Price

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-SC002868
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	CDK19
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CDK19 cyclin-dependent kinase 19 [ Homo sapiens ]
Gene Symbol	CDC2L6
Synonyms	CDK11; CDC2L6; bA346C16.3
Gene Description	cell division cycle 2-like 6 (CDK8-like)
GeneID	23097
Uni ProtID	Q9BWU1
mRNA Refseq	NM_015076.3
Protein Refseq	NP_055891.1
Chromosome Location	6q21
Function	ATP binding; cyclin-dependent protein kinase activity;
Pathway	Developmental Biology, organism-specific biosystem; Fatty acid, triacylglycerol, and ketone body metabolism, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of lipids and lipoproteins, organism-specific biosystem; PPARA Activates Gene Expression, organism-specific biosystem; Regulation of Lipid Metabolism by Peroxisome proliferator-activated receptor alpha (PPARalpha), organism-specific biosystem; Transcriptional Regulation of White Adipocyte Differentiation, organism-specific biosystem;
MIM	614720

Quick Inquiry

Case Study

Q & A

Customer Reviews

Previous studies have shown that miR-222-3p is a tumor-suppressive miRNA in ovarian cancer (OC). Here, researchers aimed to further understand the regulatory role of miR-222-3p in ovarian cancer and provide new mechanisms for its prevention and treatment. The researchers found that miR-222-3p inhibits the migration and proliferation of ovarian cancer cells. CDK19 is highly expressed in ovarian cancer and is negatively correlated with miR-222-3p. Furthermore, miR-222-3p directly binds to the 3′-UTR of CDK19 and inhibits its translation, thereby inhibiting the migration and proliferation of ovarian cancer cells in vitro and inhibiting tumor growth in vivo. They also observed that Hotair inhibits miR-222-3p in ovarian cancer. In addition, Hotair can promote the proliferation and migration of ovarian cancer cells in vitro and promote tumor growth and metastasis in vivo. Moreover, the expression of Hotair is positively correlated with the expression of CDK19. These results indicate that Hotair indirectly upregulates the expression of CDK19 by "sponging" miR-222-3p, thereby enhancing the malignant behavior of ovarian cancer.

Here, researchers investigated the function of CDK19. Both colony formation assays (Figure 1I) and EdU assays showed a significant increase in cell proliferation in CDK19 overexpressing OVCAR3 cells. To determine whether miR-222-3p inhibits ovarian cancer cell proliferation by targeting CDK19, the researchers designed the following rescue experiment. In both colony formation assays and EdU assays, overexpression of CDK19 abolished the inhibitory effect of miR-222-3p mimics on OVCAR3 cell proliferation (Figure 1J, K). As expected, colony formation assays and flow cytometry experiments showed that overexpression of CDK19 could also abolish the inhibitory effect of miR-222-3p mimics on HO-8910 PM cell proliferation. Furthermore, compared to the control group, the group co-transfected with miR-ctrl mimics and CDK19 showed higher levels of CDK19 mRNA and protein. Similarly, the CDK19 mRNA and protein levels, which were suppressed by miR-222-3p mimics, were effectively restored in CDK19-overexpressing HO-8910 PM cells.

Figure 1. miR-222-3p inhibits ovarian cancer cell proliferation by targeting CDK19. (Fan L, et al., 2022)

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Question *

Name

Country *

Email *

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Product Name *

Catalog Name *

Rating

Needs improvement

Satisfaction

General satisfaction

Very satisfaction

Product Review Title *

Summary *