Cat.No. : AD00359Z
Titer: ≥1x10^10 IFU/mL / ≥1x10^11 IFU/mL / ≥1x10^11 VP/mL / ≥1x10^12 VP/mL Size: 100 ul/500 ul/1 mL
Storage: -80℃ Shipping: Frozen on dry ice
| Cat. No. | AD00359Z |
| Description | Human Adenovirus Type5 (dE1/E3) expressing MDM2 with C-terminus V5 epitope tag under CMV promoter. C-terminus V5 epitope tag, pre-made adenovirus, ready to ship and ready to use format. |
| Target Gene | MDM2 |
| Product Type | Adenoviral particle |
| Insert | MDM2, C-fusion with V5 tag |
| Titer | Varies lot by lot, for example, ≥1x10^10 IFU/mL, ≥1x10^11 IFU/mL, ≥1x10^11 VP/mL etc. |
| Size | Varies lot by lot, for example, 250 ul, 500 ul, 1 mL etc. |
| Storage | Store at -80℃. Avoid multiple freeze/thaw cycles. |
| Shipping | Frozen on dry ice |
| Creative Biogene ensures high-quality adenovirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between adenovirus particle lots. | |
| Endotoxin | Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in adenovirus production, especially for applications in animal studies and gene therapy. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced adenovirus particles to ensure regulatory compliance. |
| Sterility | Creative Biogene ensures that adenovirus products are free of any bacterial, fungal and other microbial contamination. |
| Ad5 E1 Detection | All Creative Biogene adenoviruses are PCR tested to ensure that there are no detectable E1 sequences in the particles, which could be from revertants or external E1 contamination. |
| RCA Assays | Adenovirus products originating at Creative Biogene are guaranteed to have undetectable replication-competent adenovirus (RCA). This quality control measure is important because there is always the possibility of wild-type contamination due to revertants or environmental sources. |
| PFU Titering | All purified adenovirus preparations are tested for infectious titer. Creative Biogene's PFU test takes a few days longer but counts true plaques in HEK cells rather than estimating PFU titers via IHC staining or TCI50 of infected cells. |
Vascular calcification (VC) is often associated with cardiovascular and metabolic diseases. However, the molecular mechanisms linking VC to these diseases remain unclear. Here, researchers report that MDM2-induced ubiquitination of histone deacetylase 1 (HDAC1) mediates VC. Loss of HDAC1 activity by chemical inhibitors or genetic ablation enhances VC. HDAC1 protein, but not mRNA, is reduced in cellular and animal calcification models and in human calcified coronary arteries. Under calcification-inducing conditions, proteasomal degradation of HDAC1 precedes VC, mediated by the MDM2 E3 ubiquitin ligase that initiates HDAC1 K74 ubiquitination. MDM2 overexpression enhances VC, whereas MDM2 depletion attenuates VC. A decoy peptide spanning HDAC1 K74 and the MDM2 inhibitor RG 7112 inhibit VC in vitro and in vivo. These results reveal a previously unappreciated ubiquitination pathway and suggest that MDM2-mediated HDAC1 ubiquitination is a novel therapeutic target for VC.
The researchers investigated whether MDM2 could induce VC. MDM2 adenovirus (Ad-MDM2) infection induced calcium deposition in RVSMCs only at higher doses (the fourth column in Figure 1a). However, under Pi treatment, Ad-MDM2 significantly enhanced calcium deposition in RVSMCs in a dose-dependent manner (the fifth to eighth columns in Figure 1a). In addition, MDM2 siRNA significantly attenuated Pi-induced calcium deposition in A10 cells (Figure 1b).
Figure 1. (a) Adenoviral infection of MDM2 enhanced Pi-induced VC in a dose-dependent manner. (b) MDM2 siRNA blunted Pi-induced VC in A10 cells. (Kwon D H, et al., 2016)
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
The viral titer was high, and transduction efficiency in our cell lines was impressive. Highly recommended for anyone exploring p53 pathways!
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.
Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
Copyright © Creative Biogene. All rights reserved.
